{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:10:51Z","timestamp":1790601051930,"version":"4.1.0"},"reference-count":107,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2023,5,29]],"date-time":"2023-05-29T00:00:00Z","timestamp":1685318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cardiac arrest (CA) can result in cerebral ischaemia\u2013reperfusion injury and poor neurological outcomes. While bone marrow\u2010derived mesenchymal stem cells (BMSCs) have been shown to have protective effects in brain ischaemic disease, their efficacy can be reduced by the poor oxygen environment. In this study, we investigated the neuroprotective effects of hypoxic preconditioned BMSCs (HP\u2010BMSCs) and normoxic BMSCs (N\u2010BMSCs) in a cardiac arrest rat model by examining their ability to ameliorate cell pyroptosis. The mechanism underlying the process was also explored. Cardiac arrest was induced in rats for 8\u2009min and surviving rats received 1\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    normoxic\/hypoxic BMSCs or PBS via intracerebroventricular (ICV) transplantation. Neurological function of rats was evaluated using neurological deficit scores (NDSs) and examined for brain pathology. Serum S100B and neuron\u2010specific enolase (NSE) levels and cortical proinflammatory cytokines were measured to evaluate brain injury. Pyroptosis\u2010related proteins in the cortex after cardiopulmonary resuscitation (CPR) were measured using western blotting and immunofluorescent staining. Transplanted BMSCs were tracked using bioluminescence imaging. Results showed significantly better neurological function and neuropathological damage after transplantation with HP\u2010BMSCs. In addition, HP\u2010BMSCs reduced levels of pyroptosis\u2010related proteins in the rat cortex after CPR and significantly reduced levels of biomarkers for brain injury. Mechanistically, HP\u2010BMSCs alleviated brain injury by reducing the expressions of HMGB1, TLR4, NF\u2010\u03baB p65, p38 MAPK and JNK in the cortex. Our study demonstrated that hypoxic preconditioning could enhance the efficacy of BMSCs in alleviating post\u2010resuscitation cortical pyroptosis. This effect may be related to the regulation of the HMGB1\/TLR4\/NF\u2010\u03baB, MAPK signalling pathways.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.17782","type":"journal-article","created":{"date-parts":[[2023,5,29]],"date-time":"2023-05-29T08:26:15Z","timestamp":1685348775000},"page":"1836-1858","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3714-0421","authenticated-orcid":false,"given":"Xiahong","family":"Tang","sequence":"first","affiliation":[{"name":"Shengli Clinical Medical College of Fujian Medical University Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Ke","sequence":"additional","affiliation":[{"name":"Shengli Clinical Medical College of Fujian Medical University Fujian Medical University  Fuzhou China"},{"name":"Department of Emergency Fujian Provincial Hospital  Fuzhou China"},{"name":"Fujian Provincial Key Laboratory of Emergency Medicine  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Falu","family":"Chen","sequence":"additional","affiliation":[{"name":"Shengli Clinical Medical College of Fujian Medical University Fujian Medical University  Fuzhou China"},{"name":"Department of Emergency Fujian Provincial Hospital  Fuzhou China"},{"name":"Fujian Provincial Key Laboratory of Emergency Medicine  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingming","family":"Lin","sequence":"additional","affiliation":[{"name":"Shengli Clinical Medical College of Fujian Medical University Fujian Medical University  Fuzhou China"},{"name":"Department of Emergency Fujian Provincial Hospital  Fuzhou China"},{"name":"Fujian Provincial Key Laboratory of Emergency Medicine  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"You","sequence":"additional","affiliation":[{"name":"The Second Department of Intensive Care Unit Fujian Provincial Hospital South Branch  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shengli Clinical Medical College of Fujian Medical University Fujian Medical University  Fuzhou China"},{"name":"Department of Emergency Fujian Provincial Hospital  Fuzhou China"},{"name":"Fujian Provincial Key Laboratory of Emergency Medicine  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Gong","sequence":"additional","affiliation":[{"name":"Shengli Clinical Medical College of Fujian Medical University Fujian Medical University  Fuzhou China"},{"name":"Department of Emergency Fujian Provincial Hospital  Fuzhou China"},{"name":"Fujian Provincial Key Laboratory of Emergency Medicine  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Han","sequence":"additional","affiliation":[{"name":"Shengli Clinical Medical College of Fujian Medical University Fujian Medical University  Fuzhou China"},{"name":"Department of Emergency Fujian Provincial Hospital  Fuzhou China"},{"name":"Fujian Provincial Key Laboratory of Emergency Medicine  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangping","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Shengli Clinical Medical College of Fujian Medical University Fujian Medical University  Fuzhou China"},{"name":"Department of Emergency Fujian Provincial Hospital  Fuzhou China"},{"name":"Fujian Provincial Key Laboratory of Emergency Medicine  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Chen","sequence":"additional","affiliation":[{"name":"Shengli Clinical Medical College of Fujian Medical University Fujian Medical University  Fuzhou China"},{"name":"Department of Emergency Fujian Provincial Hospital  Fuzhou China"},{"name":"Fujian Provincial Key Laboratory of Emergency Medicine  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncl.2017.06.001"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)30472-0"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2019.1696"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.190652"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-004-2425-z"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000558"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9572(97)00035-X"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1109766"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.11137"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000256294.46009.29"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116618"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(98)00024-0"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-020-01879-1"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12950-020-00255-3"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1960"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/9743280"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12414"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-016-0243-4"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-021-00670-3"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-021-03511-x"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2019.172487"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.540669"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.107032"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.17-0129"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.46436"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2614"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-015-0284-x"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/09636897211024183"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2018.08.004"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2017.07.004"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2021.09.001"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.184"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e31818a8ff0"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.265563"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2021.113700"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2021.641157"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.00182"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.239848"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2014.09.033"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368912X657251"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18061320"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-020-00868-5"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-016-0445-1"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2007.00336.x"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-1104"},{"issue":"5","key":"e_1_2_11_47_1","first-page":"4312","article-title":"Bone marrow mesenchymal stem cells repair the hippocampal neurons and increase the expression of IGF\u20101 after cardiac arrest in rats","volume":"14","author":"Tang X","year":"2017","journal-title":"Exp Ther Med"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-2457(00)00379-5"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-021-06481-4"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2006.06.040"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2005.04.015"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccc.2020.07.005"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2017.01.024"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11010131"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/joim.13260"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000757"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000023891.80661.AD"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1213\/ane.0b013e318193ca99"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-019-0209-z"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12534"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature15632"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18629"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112051"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2021.114585"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-018-0294-7"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116599"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2019.01.003"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2020.577927"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-021-02233-9"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-022-01068-4"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202002944"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2019.03.003"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22020763"},{"key":"e_1_2_11_74_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-02011-z"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.793829"},{"issue":"2","key":"e_1_2_11_76_1","first-page":"378","article-title":"Preconditioning of stem cells for the treatment of myocardial infarction","volume":"125","author":"Lu HH","year":"2012","journal-title":"Chin Med J (Engl)"},{"key":"e_1_2_11_77_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2763"},{"key":"e_1_2_11_78_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells7050036"},{"key":"e_1_2_11_79_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000084828.50310.6A"},{"key":"e_1_2_11_80_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-018-0802-8"},{"key":"e_1_2_11_81_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566523221666210707125342"},{"key":"e_1_2_11_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2020.01.029"},{"key":"e_1_2_11_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2018.02.001"},{"key":"e_1_2_11_84_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2020.0174"},{"key":"e_1_2_11_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2013.11.002"},{"key":"e_1_2_11_86_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-020-00815-2"},{"key":"e_1_2_11_87_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00252-12"},{"key":"e_1_2_11_88_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.103307"},{"key":"e_1_2_11_89_1","doi-asserted-by":"publisher","DOI":"10.1159\/000485490"},{"key":"e_1_2_11_90_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.202807"},{"key":"e_1_2_11_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2012.09.030"},{"key":"e_1_2_11_92_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-43242-2"},{"key":"e_1_2_11_93_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0665-z"},{"key":"e_1_2_11_94_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-012-2118-2"},{"key":"e_1_2_11_95_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12657"},{"key":"e_1_2_11_96_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1304-z"},{"key":"e_1_2_11_97_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/7450151"},{"key":"e_1_2_11_98_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/434692"},{"key":"e_1_2_11_99_1","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S341581"},{"key":"e_1_2_11_100_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7879629"},{"key":"e_1_2_11_101_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-12-86"},{"key":"e_1_2_11_102_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20184658"},{"key":"e_1_2_11_103_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.24025"},{"key":"e_1_2_11_104_1","doi-asserted-by":"publisher","DOI":"10.3390\/md18020116"},{"key":"e_1_2_11_105_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-015-0157-3"},{"key":"e_1_2_11_106_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-021-03375-1"},{"key":"e_1_2_11_107_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/1552127"},{"key":"e_1_2_11_108_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.9664"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.17782","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T09:57:55Z","timestamp":1692266275000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.17782"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,29]]},"references-count":107,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.1111\/jcmm.17782"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.17782","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,29]]},"assertion":[{"value":"2023-01-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}