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Patients diagnosed with AMkL have low overall survival and have poor outcome to treatment, thus novel therapies such as CAR T cell therapy could represent an alternative in treating AMkL. We investigated the effect of a new CAR T cell which targets CD41, a specific surface antigen for M7\u2010AMkL, against an in vitro model for AMkL, DAMI Luc2 cell line. The performed flow cytometry evaluation highlighted a percentage of 93.8% CAR T cells eGFP\u2010positive and a limited acute effect on lowering the target cell population. However, the interaction between effector and target (E:T) cells, at a low ratio, lowered the cell membrane integrity, and reduced the M7\u2010AMkL cell population after 24\u2009h of co\u2010culture, while the cytotoxic effect was not significant in groups with higher E:T ratio. Our findings suggest that the anti\u2010CD41 CAR T cells are efficient for a limited time spawn and the cytotoxic effect is visible in all experimental groups with low E:T ratio.<\/jats:p>","DOI":"10.1111\/jcmm.17810","type":"journal-article","created":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T05:49:27Z","timestamp":1693892967000},"page":"2864-2875","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Design and preclinical testing of an <scp>anti\u2010CD41 CAR<\/scp> T cell for the treatment of acute megakaryoblastic leukaemia"],"prefix":"10.1111","volume":"27","author":[{"given":"Adrian Bogdan","family":"Tigu","sequence":"first","affiliation":[{"name":"Medfuture Research Center for Advanced Medicine Iuliu Hatieganu University of Medicine and Pharmacy  Cluj\u2010Napoca Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catalin 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