{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T07:07:02Z","timestamp":1789801622984,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:00:00Z","timestamp":1690329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2021M690990"],"award-info":[{"award-number":["2021M690990"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Prolonged exposure of the peritoneum to high glucose dialysate leads to the development of peritoneal fibrosis (PF), and apoptosis of peritoneal mesothelial cells (PMCs) is a major cause of PF. The aim of this study is to investigate whether Astragaloside IV could protect PMCs from apoptosis and alleviate PF. PMCs and rats PF models were induced by high glucose peritoneal fluid. We examined the pathology of rat peritoneal tissue by HE staining, the thickness of rat peritoneal tissue by Masson's staining, the number of mitochondria and oxidative stress levels in peritoneal tissue by JC\u20101 and DHE fluorescence staining, and mitochondria\u2010related proteins and apoptosis\u2010related proteins such as PGC\u20101\u03b1, NRF1, TFAM, Caspase3, Bcl2 smad2 were measured. We used hoechst staining and flow cytometry to assess the apoptotic rate of PMCs in the PF model, and further validated the observed changes in the expressions of PGC\u20101\u03b1, NRF1, TFAM, Caspase3, Bcl2 smad2 in PMCs. We further incubated PMCs with MG\u2010132 (proteasome inhibitor) and Cyclohexylamine (protein synthesis inhibitor). The results demonstrated that Astragaloside IV increased the expression of PGC\u20101\u03b1 by reducing the ubiquitination of PGC\u20101\u03b1. It was further found that the protective effects of Astragaloside IV on PMCs were blocked when PGC\u20101\u03b1 was inhibited. In conclusion, Astragaloside IV effectively alleviated PF both in vitro and in vivo, possibly by promoting PGC\u20101\u03b1 to enhance mitochondrial synthesis to reduce apoptotic effects.<\/jats:p>","DOI":"10.1111\/jcmm.17871","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T17:24:53Z","timestamp":1690392293000},"page":"2945-2955","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Astragaloside <scp>IV<\/scp> ameliorates peritoneal fibrosis by promoting <scp>PGC<\/scp>\u20101\u03b1 to reduce apoptosis in vitro and in vivo"],"prefix":"10.1111","volume":"27","author":[{"given":"Mingxia","family":"Xie","sequence":"first","affiliation":[{"name":"College of Clinical Medicine Hunan University of Chinese Medicine  Changsha People's Republic of China"},{"name":"College of Traditional Chinese Medicine Hunan University of Chinese Medicine  Changsha People's Republic of 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