{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:48:47Z","timestamp":1776732527760,"version":"3.51.2"},"reference-count":26,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2023,8,15]],"date-time":"2023-08-15T00:00:00Z","timestamp":1692057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It has been reported that Banxia\u2010houpo decoction (BXHPD) serves as the anti\u2010depressant treatment for a mild and severe depressive disease with limited side effects. The present study was performed to evaluate the protective effect of BXHPD on chronic unpredicted mild stress (CUMS)\u2010induced depression and explore its effect on TrkA\/Akt\u2010mediated microglia polarization. The CUMS procedure was carried out, and the mice were intragastrically treated with BXHPD once daily. The selective TrkA inhibitor GW441756 was applied to further investigate the role of TrkA in BXHPD\u2010mediated microglia polarization. The behaviour test including open field test (OFT), sucrose preference test (SPT), novelty\u2010suppressed feeding test (NSFT), tail suspension test (TST) and forced swim test (FST) was performed. The concentrations of pro\u2010inflammatory cytokines IL\u20106, TNF\u2010\u03b1, IL\u20101\u03b2, IL\u201012 and anti\u2010inflammatory cytokines IL\u20104, IL\u201010 were determined using Enzyme\u2010linked immunosorbent assay. The population of Iba1+ cells and the length of microglia processes were observed under the fluorescence microscope. The mRNA expressions of Arg1, Ym1 and Fizzl1 were measured by PCR. The protein expressions of TrkA, p\u2010Tyr490\u2010TrkA, p\u2010Ser473\u2010Akt, p\u2010Ser473\u2010Akt1, p\u2010Ser474\u2010Akt2, p\u2010CREB and Jmjd3 were detected by western blot. Our results showed that BXHPD attenuated CUMS\u2010induced depressive\u2010like behaviour, promoted anti\u2010inflammatory cytokines, inhibited pro\u2010inflammatory cytokines, suppressed microglia activation, promoted M2 phenotype\u2010specific indices and upregulated the expressions of TrkA, p\u2010Tyr490\u2010TrkA, p\u2010Ser473\u2010Akt, p\u2010Ser473\u2010Akt1, p\u2010Ser474\u2010Akt2, p\u2010CREB and Jmjd3. The above beneficial effect of BXHPD can be blocked by TrkA inhibitor GW441756. This work demonstrated that BXHPD exerted an anti\u2010depressant effect by promoting M2 phenotype microglia polarization via TrkA\/Akt pathway.<\/jats:p>","DOI":"10.1111\/jcmm.17906","type":"journal-article","created":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T12:44:54Z","timestamp":1692362694000},"page":"3339-3353","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["The effect of Banxia\u2010houpo decoction on CUMS\u2010induced depression by promoting M2 microglia polarization via TrkA\/Akt signalling"],"prefix":"10.1111","volume":"27","author":[{"given":"Li","family":"Liu","sequence":"first","affiliation":[{"name":"School of Pharmacy Guangdong Medical University  Dongguan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Neurology Department Kunshan Hospital Affiliated to Nanjing University of Chinese Medicine  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