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First, differentially expressed long non\u2010coding RNAs (lncRNAs) from EVs separated from serum between BCS and healthy controls were screened using microarray analysis. Second, the proliferation, migration and tube formation of human vascular endothelial cells (HUVECs) were detected after EVs treatment, along with vascular endothelial growth factor (VEGF) levels and inflammatory factors from the cell supernatant. Last, the overexpressed lncRNA was transfected into the cells to further explore the mechanisms involved. Extracellular vesicles of BCS patients have significantly higher levels of lncRNA MTUS2\u20105 than healthy controls. Apparently, treatment with EVs from BCS or the ones transfected with plasmids that overexpress lncRNA MTUS2\u20105 enhances proliferation, migration and angiogenesis capacity. The results were considerably better than those obtained from treatment with EVs from healthy controls or transfection with the normal control plasmid, which also elevated the level of VEGF and inflammatory factors. Furthermore, FOS and PTGS2 were potentially regulated by the lncRNA MTUS2\u20105 transmitted by EVs. The lncRNA MTUS2\u20105 in EVs plays an important role in angiogenesis in the Budd\u2013Chiari syndrome.<\/jats:p>","DOI":"10.1111\/jcmm.17911","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T19:36:15Z","timestamp":1692646575000},"page":"3431-3442","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Extracellular vesicles\u2010transmitted long non\u2010coding RNA MTUS2\u20105 promotes proliferation and vascularization of human vascular endothelial cells in patients with Budd\u2013Chiari syndrome"],"prefix":"10.1111","volume":"27","author":[{"given":"Longfei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Vascular Surgery The First Affiliated Hospital of Bengbu Medical College  Bengbu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benchi","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery 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