{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:39:00Z","timestamp":1790138340738,"version":"4.0.1"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T00:00:00Z","timestamp":1705363200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81903013"],"award-info":[{"award-number":["81903013"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Understanding the mechanisms underlying metastasis in hepatocellular carcinoma (HCC) is crucial for developing new therapies against this fatal disease. Deubiquitinase ubiquitin\u2010specific protease 11 (USP11) belongs to the deubiquitinating family and has previously been reported to play a critical role in cancer pathogenesis. Although it has been established that USP11 can facilitate the metastasis and proliferation ability of HCC, the underlying regulatory mechanisms are poorly understood. The primary objective of this research was to reveal hitherto undocumented functions of USP11 during HCC progression, especially those related to metabolism. Under hypoxic conditions, USP11 was found to significantly impact the glycolysis of HCC cells, as demonstrated through various techniques, including RNA\u2010Seq, migration and colony formation assays, EdU and co\u2010immunoprecipitation. Interestingly, we found that USP11 interacted with the HIF\u20101\u03b1 complex and maintained HIF\u20101\u03b1 protein stability by removing ubiquitin. Moreover, USP11\/HIF\u20101\u03b1 could promote glycolysis through the PDK1 and LDHA pathways. In general, our results demonstrate that USP11 promotes HCC proliferation and metastasis through HIF\u20101\u03b1\/LDHA\u2010induced glycolysis, providing new insights and the experimental basis for developing new treatments for this patient population.<\/jats:p>","DOI":"10.1111\/jcmm.18017","type":"journal-article","created":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T06:50:19Z","timestamp":1705474219000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<scp>USP11<\/scp> promotes glycolysis by regulating <scp>HIF<\/scp>\u20101\u03b1 stability in hepatocellular carcinoma"],"prefix":"10.1111","volume":"28","author":[{"given":"Lijun","family":"Qiao","sequence":"first","affiliation":[{"name":"College of Pharmacy, Shenzhen Technology University  Shenzhen Guangdong China"},{"name":"Department of Hepatobiliary and Pancreas Surgery The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital)  Shenzhen Guangdong China"},{"name":"Department of Hepatobiliary and Pancreas Surgery, The First Affiliated Hospital Southern University of Science and Technology  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weibin","family":"Hu","sequence":"additional","affiliation":[{"name":"Institute for Brain Research and Rehabilitation, South China Normal University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linzhi","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Shenzhen Technology University  Shenzhen Guangdong 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