{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:17:23Z","timestamp":1792019843924,"version":"4.4.0"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:00:00Z","timestamp":1700006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["08102003"],"award-info":[{"award-number":["08102003"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["5K19425"],"award-info":[{"award-number":["5K19425"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["21K16344"],"award-info":[{"award-number":["21K16344"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["21K15375"],"award-info":[{"award-number":["21K15375"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sleep apnea syndrome (SAS) exposes cells throughout the body to intermittent hypoxia (IH). Intermittent hypoxia is a risk factor not only for hypertension and insulin resistance but also for vascular dysfunction. We have reported correlations between IH, insulin resistance and hypertension. However, the details of why IH leads to vascular dysfunction remain unclear. In this study, we investigated inflammation\u2010related transcripts in vascular endothelial cells (human HUEhT\u20101 and mouse UV2) exposed to IH by real\u2010time RT\u2010PCR and found that <jats:italic>intercellular adhesion molecule\u20101<\/jats:italic> (<jats:italic>ICAM\u20101<\/jats:italic>) and <jats:italic>endothelial cell\u2010specific molecule\u20101<\/jats:italic> (<jats:italic>ESM1<\/jats:italic>) mRNAs were significantly increased. ELISA confirmed that, in the UV2 cell medium, ICAM\u20101 and ESM1 were significantly increased by IH. However, the promoter activities of <jats:italic>ICAM\u20101<\/jats:italic> and <jats:italic>ESM1<\/jats:italic> were not upregulated. On the other hand, IH treatment significantly decreased microRNA (miR)\u2010181a1 in IH\u2010treated cells. The introduction of miR\u2010181a1 mimic but not miR\u2010181a1 mimic NC abolished the IH\u2010induced upregulation of Ican\u20101 and ESM1. These results indicated that ICAM\u20101 and ESM1 were upregulated by IH via the IH\u2010induced downregulation of miR\u2010181a1 in vascular endothelial cells and suggested that SAS patients developed atherosclerosis via the IH\u2010induced upregulation of ICAM\u20101 and ESM1.<\/jats:p>","DOI":"10.1111\/jcmm.18039","type":"journal-article","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T00:39:59Z","timestamp":1700095199000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Intermittent hypoxia increased the expression of <scp>ESM1<\/scp> and <scp>ICAM\u20101<\/scp> in vascular endothelial cells via the downregulation of <scp>microRNA<\/scp>\u2010181a1"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4066-0199","authenticated-orcid":false,"given":"Shin","family":"Takasawa","sequence":"first","affiliation":[{"name":"Department of Biochemistry Nara Medical University  Nara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Makino","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Nara Medical University  Nara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiyo","family":"Yamauchi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Nara Medical University  Nara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumiyo","family":"Sakuramoto\u2010Tsuchida","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Nara Medical University  Nara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rina","family":"Hirota","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Nara Medical University  Nara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryusei","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Nara Medical University  Nara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keito","family":"Asai","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Nara Medical University  Nara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Takeda","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Nara Medical University  Nara Japan"},{"name":"Department of Obstetrics and Gynecology Nara Medical University  Nara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Uchiyama","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Nara Medical University  Nara Japan"},{"name":"Department of Diagnostic Pathology Nara Medical University  Nara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryogo","family":"Shobatake","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Nara Medical University  Nara Japan"},{"name":"Department of Neurology Nara Medical University  Nara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyo","family":"Ota","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Nara Medical University  Nara Japan"},{"name":"Department of Respiratory Medicine Nara Medical University  Nara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00043.2008"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1273092"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.01035"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2017.05.009"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10173770"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200306-767OC"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201002-0162OC"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2014.12.012"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.96.10.3378"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.101.9.948"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/1817-1737.160840"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smrv.2014.03.004"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003319720903586"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867326666190903112146"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0891-5849(00)00223-9"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/crj.12487"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17101712"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph16173101"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20194756"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.12.112"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.20-0570"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2022.13506"},{"key":"e_1_2_9_24_1","first-page":"5526","article-title":"Establishment and characterization of a tumorigenic murine vascular endothelial cell line (F\u20102)","volume":"50","author":"Toda K","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2014.05.009"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.02.080"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2011.11.011"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2013.09.001"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2017.07.005"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms221810127"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23115868"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23158782"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17618"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01731-2016"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20215462"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17498"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11179"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.281"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1053\/smrv.2002.0261"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smrv.2014.07.003"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_202006_21685"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2007.09.010"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.100.15.1646"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000056108.73219.0A"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E16-12-0827"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00248.2006"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2015.09.030"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3109\/08037051.2014.972816"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2005.08.004"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003319713513492"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_202006_21707"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003319717736158"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/9134096"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2017.12.040"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3074"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21062092"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201602-0323OC"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.69035"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.9786"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1159\/000477389"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719118805865"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0195604"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.18039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T09:36:31Z","timestamp":1758533791000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.18039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,15]]},"references-count":61,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1111\/jcmm.18039"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.18039","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,15]]},"assertion":[{"value":"2023-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e18039"}}