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The GSE80178 dataset was acquired from the Gene Expression Omnibus (GEO) database, containing six DFU and three diabetic foot skin (DFS) samples. The limma package was used to identify differentially expressed genes (DEGs). At the same time, DEGs associated with CS (CS\u2010DEGs) were found using the CellAge database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted on the CS\u2010DEGs. A protein\u2013protein interaction (PPI) network was built using the String database, and the cytoHubba plug\u2010in within Cytoscape helped identify hub genes. Lastly, the miRNA\u2010TF\u2010mRNA regulatory network for these hub genes was established. In total, 66 CS\u2010DEGs were obtained. These genes mainly focus on CS, Kaposi sarcoma\u2010associated herpesvirus infection and Toll\u2010like receptor signalling pathway. Eight hub genes were identified to regulate cell senescence in DFU, including TP53, SRC, SIRT1, CCND1, EZH2, CXCL8, AR and CDK4. 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