{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:23:22Z","timestamp":1788305002346,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,12,25]],"date-time":"2023-12-25T00:00:00Z","timestamp":1703462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011447","name":"Science and Technology Department of Henan Province","doi-asserted-by":"publisher","award":["222102310028"],"award-info":[{"award-number":["222102310028"]}],"id":[{"id":"10.13039\/501100011447","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Endothelial dysfunction is an independent risk factor for stroke. The dysfunction of endothelial cells (EC) is closely concerned with EC senescence. Gastrodin (GAS) is an organic compound extracted from the dried root mass of the Orchidaceae plant Gastrodiae gastrodiae. It is used clinically to treat diseases such as vertebrobasilar insufficiency, vestibular neuronitis and vertigo. In the present study, we used hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    )\u2010induced human umbilical vein endothelial cells (HUVECs) to establish an in\u00a0vitro EC senescence model and to investigate the role and mechanism of GAS in EC senescence. It's found that H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010treated HUVECs increased the proportion of senescence\u2010associated \u03b2\u2010galactosidase (SA \u03b2\u2010gal) positive cells and the relative protein expression levels of senescence\u2010associated cyclin p16 and p21. In addition, GAS reduced the proportion of SA \u03b2\u2010gal positive cells and the relative protein expression levels of p16 and p21, and increased the proliferation and migration ability of HUVECs. Meanwhile, GAS increased the expression of the anti\u2010oxidative stress protein HO\u20101 and its nuclear expression level of Nrf2. The anti\u2010senescence effect of GAS was blocked when HO\u20101 expression was inhibited by SnPPIX. Furthermore, absence of HO\u20101 abolished the effect of GAS on HUVEC proliferation and migration. In conclusion, GAS ameliorated H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010induced cellular senescence and enhanced cell proliferation and migration by enhancing Nrf2\/HO\u20101 signalling in HUVECs. These findings of our study expanded the understanding of GAS pharmacology and suggested that GAS may offer a potential therapeutic agent for stroke.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.18089","type":"journal-article","created":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T02:58:20Z","timestamp":1703559500000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Gastrodin alleviates premature senescence of vascular endothelial cells by enhancing the Nrf2\/\n                    <scp>HO<\/scp>\n                    \u20101 signalling pathway"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3411-0766","authenticated-orcid":false,"given":"Pengfei","family":"Tong","sequence":"first","affiliation":[{"name":"Neurosurgery Department The Third People\u2019s Hospital of Henan Province, Zhongyuan District  Zhengzhou City Henan Province 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