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Exosomal miRNAs play significant roles in embryonic development and cellular communication. Hereon, we explored the roles of serum exosomes derived from SA patients on trophoblast EMT, migration and invasion. Exosomes were isolated from normal control (NC) patients with abortion for unplanned pregnancy and SA patients, then characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting. Exosomal miRNA profiles were identified by miRNA sequencing. The effects of serum exosomes on trophoblast migration and invasion were detected by scratch wound healing and transwell assays, and other potential mechanisms were revealed by quantitative real\u2010time PCR (RT\u2010PCR), western blotting and dual\u2010luciferase reporter assay. Finally, animal experiments were used to explore the effects of exosomal miR\u2010410\u20103p on embryo absorption in mice. The serum exosomes from SA patients inhibited trophoblast EMT and reduced their migration and invasion ability in\u00a0vitro. The miRNA sequencing showed that miR\u2010410\u20103p was upregulated in SA serum exosomes. The functional experiments showed that SA serum exosomes restrained trophoblast EMT, migration and invasion by releasing miR\u2010410\u20103p. Mechanistically, SA serum exosomal miR\u2010410\u20103p inhibited trophoblast cell EMT, migration and invasion by targeting TNF receptor\u2010associated factor 6 (TRAF6) at the post\u2010transcriptional level. Besides, SA serum exosomal miR\u2010410\u20103p inhibited the p38 MAPK signalling pathway by targeting TRAF6 in trophoblasts. Moreover, milk exosomes loaded with miR\u2010410\u20103p mimic reached the maternal\u2010fetal interface and aggravated embryo absorption in female mice. Clinically, miR\u2010410\u20103p and TRAF6 expression were abnormal and negatively correlated in the placental villi of SA patients. Our findings indicated that exosome\u2010derived miR\u2010410\u20103p plays an important role between SA serum and trophoblasts in intercellular communication, suggesting a novel mechanism by which serum exosomal miRNA regulates trophoblasts in SA patients.<\/jats:p>","DOI":"10.1111\/jcmm.18097","type":"journal-article","created":{"date-parts":[[2024,1,2]],"date-time":"2024-01-02T13:50:39Z","timestamp":1704203439000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Exosome\u2010delivered <scp>miR<\/scp>\u2010410\u20103p reverses epithelial\u2010mesenchymal transition, migration and invasion of trophoblasts in spontaneous abortion"],"prefix":"10.1111","volume":"28","author":[{"given":"Zhen\u2010yue","family":"Chen","sequence":"first","affiliation":[{"name":"The First Clinical Medical College of Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Lingnan Medical Research Center of Guangzhou 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