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However, its function remains unclear in cholangiocarcinoma (CCA). Here, we evaluated the impact AZGP1 in CCA using Gene Expression Omnibus (GEO) and GEPIA. In addition, we analysed AZGP1 expression using quantitative reverse transcription PCR and western blotting. Expression of AZGP1 was nearly deficient in CCA patients and cell lines and was associated with poor prognosis. AZGP1 overexpression upregulated apoptosis markers. Co\u2010immunoprecipitation experiments showed that AZGP1 interacts with tripartite motif\u2010containing protein 25 (TRIM25), and tissue microarray and bioinformatic analysis showed that AZGP1 is negatively correlated with TRIM25 expression in CCA. Thereafter, TRIM25 knockdown led to AZGP1 upregulation and induced cancer cell apoptosis. TRIM25 targets AZGP1 for degradation by catalysing its ubiquitination. AZGP1 overexpression significantly suppressed tumour growth in a xenograft mouse model. This study findings suggest that AZGP1 is a potential therapeutic target or a diagnostic biomarker for treating patients with CCA.<\/jats:p>","DOI":"10.1111\/jcmm.18104","type":"journal-article","created":{"date-parts":[[2024,1,6]],"date-time":"2024-01-06T12:49:34Z","timestamp":1704545374000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Degradation of <scp>AZGP1<\/scp> suppresses apoptosis and facilitates cholangiocarcinoma tumorigenesis via <scp>TRIM25<\/scp>"],"prefix":"10.1111","volume":"28","author":[{"given":"Hyeseon","family":"Yun","sequence":"first","affiliation":[{"name":"Asan Institute for Life Science Asan Medical Center  Seoul Korea"},{"name":"Department of Pharmacology, AMIST, Asan Medical Center University of Ulsan College of Medicine  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Rae","family":"Jeong","sequence":"additional","affiliation":[{"name":"Asan 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