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We dissected the transcriptomics after pancreatic trauma and ucMSC\u2010Ex therapy by high\u2010throughput sequencing. Additionally, we used rapamycin and MHY1485 to regulate mTOR. HE, inflammatory factors and pancreatic enzymatic assays were used to comprehensively determine the local versus systemic injury level, fluorescence staining and electron microscopy were used to detect the effect of autophagy, and observe the expression levels of autophagy\u2010related markers at the gene and protein levels. High\u2010throughput sequencing identified that autophagy played a crucial role in the pathophysiological process of TP and ucMSC\u2010Ex therapy. The results of electron microscopy, immunofluorescence staining, polymerase chain reaction and western blot suggested that therapeutic effect of ucMSC\u2010Ex was mediated by activation of autophagy in pancreatic acinar cells through inhibition of mTOR. ucMSC\u2010Ex can attenuate pancreas injury by inhibiting mTOR to regulate acinar cell autophagy after TP. Future studies will build on the comprehensive sequencing of RNA carried by ucMSC\u2010Ex to predict and verify specific non\u2010coding RNA.<\/jats:p>","DOI":"10.1111\/jcmm.18120","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T12:11:25Z","timestamp":1707999085000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Still water run deep: Therapeutic <scp>TP<\/scp> effect of <scp>ucMSC\u2010Ex<\/scp> via regulating <scp>mTOR<\/scp> to enhance autophagy"],"prefix":"10.1111","volume":"28","author":[{"given":"Zhirong","family":"Zhao","sequence":"first","affiliation":[{"name":"Research Institute of General Surgery Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Han","sequence":"additional","affiliation":[{"name":"General Surgery Center General Hospital of Western Theater Command  Chengdu Sichuan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Xin","sequence":"additional","affiliation":[{"name":"General Surgery Center General Hospital of Western Theater Command  Chengdu Sichuan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lichen","family":"Zhou","sequence":"additional","affiliation":[{"name":"General Surgery Center General Hospital of Western Theater Command  Chengdu Sichuan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kexin","family":"Jiang","sequence":"additional","affiliation":[{"name":"General Surgery Center General Hospital of Western Theater Command  Chengdu Sichuan Province China"},{"name":"College of Medicine Southwest Jiaotong University  Chengdu Sichuan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7362-1927","authenticated-orcid":false,"given":"Qian","family":"Huang","sequence":"additional","affiliation":[{"name":"Research Institute of General Surgery Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5487-135X","authenticated-orcid":false,"given":"Ruiwu","family":"Dai","sequence":"additional","affiliation":[{"name":"General Surgery Center General Hospital of Western Theater Command  Chengdu Sichuan Province China"},{"name":"College of Medicine Southwest Jiaotong University  Chengdu Sichuan Province China"},{"name":"Clinical Medical College Chengdu Medical College  Chengdu Sichuan Province China"},{"name":"Pancreatic injury and repair Key laboratory of Sichuan Province General Hospital of Western Theater Command  Chengdu Sichuan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00261-019-02241-7"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0b013e318250ad07"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.09.066"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-022-02893-1"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-022-01732-1"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-022-02826-y"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fob.2014.07.006"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/bf01391745"},{"key":"e_1_2_11_10_1","first-page":"367","article-title":"Human acute pancreatitis: a light and electron microscopic study. 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