{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T17:02:32Z","timestamp":1792256552468,"version":"4.4.0"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Peripheral arterial disease (PAD) is an increasing cause of morbidity and its severity is graded based on clinical manifestation. To investigate the influence of the different stages on myopathy of ischemic muscle we analysed severity\u2010dependent effects of mitochondrial respiration in PAD. Eighteen patients with severe PAD, defined as chronic limb\u2010threatening ischemia, 47 patients with intermittent claudication (IC) and 22 non\u2010ischemic controls were analysed. High\u2010resolution respirometry (HRR) was performed on muscle biopsies of gastrocnemius and vastus lateralis muscle of patients in different PAD stages to investigate different respiratory states. Results from HRR are given as median and interquartile range and were normalized to citrate synthase activity (CSA), a marker for mitochondrial content. In order to account for inter\u2010individual differences between patients and controls, we calculated the ratio of O\u2082\u2010flux in gastrocnemius muscle over vastus muscle (\u2018GV ratio\u2019). CSA of the gastrocnemius muscle as a proxy for mitochondrial content was significantly lower in critical ischemia compared to controls. Mitochondrial respiration normalized to CSA was higher in IC compared to controls. Likewise, the GV ratio was significantly higher in IC compared to control. Mitochondrial respiration and CSA of PAD patients showed stage\u2010dependent modifications with greater changes in the mild PAD stage group (IC).<\/jats:p>","DOI":"10.1111\/jcmm.18126","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T13:34:37Z","timestamp":1711546477000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mitochondrial respiration in peripheral arterial disease depends on stage severity"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0048-0943","authenticated-orcid":false,"given":"Fiona","family":"Speichinger","sequence":"first","affiliation":[{"name":"Department of Vascular Surgery Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"},{"name":"Department of General and Visceral Surgery Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Gratl","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"},{"name":"Department of Vascular Surgery Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Raude","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"Schawe","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Carstens","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina A.","family":"Hering","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"},{"name":"Department of General and Visceral Surgery Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Greiner","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominik","family":"Pesta","sequence":"additional","affiliation":[{"name":"Institute of Aerospace Medicine German Aerospace Center (DLR)  Cologne Germany"},{"name":"Centre for Endocrinology, Diabetes and Preventive Medicine (CEDP) University Hospital Cologne  Cologne Germany"},{"name":"Cologne Excellence Cluster on Cellular Stress Responses in Aging\u2010Associated Diseases (CECAD)  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan Paul","family":"Frese","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/life12071041"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(19)30293-1"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0741-5214(97)70045-4"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejvs.2017.07.005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejvs.2019.05.006"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9121304"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.122.322325"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.123.322913"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/1538574407311106"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0741-5214(03)00602-5"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejvs.2019.08.011"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-021-02908-0"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2006.04.003"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1067\/mhj.2000.103934"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.123235"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP086905"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00690.2021"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-382-0_3"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.230185"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/ACM.0b013e3181ccd618"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.120.313831"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/1538574408315995"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365517509095787"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9030570"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)61249-0"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1984.56.4.831"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1992.73.5.2004"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00285.2011"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.90374.2008"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2005.09.065"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.303517"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.23736\/S0392-9590.17.03824-X"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.87.2.413"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005768-200112000-00007"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2004.01.015"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00141.2011"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-11-230"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2015.02.011"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00234-006-0183-z"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00024.2007"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trre.2011.10.006"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2013.00095"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00356.2015"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00141"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2016.12.106"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-017-1232-6"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00595.2018"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.18126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,27]],"date-time":"2024-04-27T04:47:56Z","timestamp":1714193276000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.18126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,27]]},"references-count":47,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1111\/jcmm.18126"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.18126","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,27]]},"assertion":[{"value":"2023-09-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e18126"}}