{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T22:40:05Z","timestamp":1785192005330,"version":"3.55.0"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,3]]},"abstract":"Abstract<\/jats:title>Acne vulgaris represents a chronic inflammatory condition, the pathogenesis of which is closely associated with the altered skin microbiome. Recent studies have implicated a profound role of Gram\u2010negative bacteria in acne development, but there is a lack of antiacne agents targeting these bacteria. Polyphyllins are major components of Rhizoma Paridis with great anti\u2010inflammatory potential. In this study, we aimed to evaluate the antiacne effects and the underlying mechanisms of PPH and a PPH\u2010enriched Rhizoma Paridis extract (RPE) in treating the Gram\u2010negative bacteria\u2010induced acne. PPH and RPE treatments significantly suppressed the mRNA and protein expressions of interleukin (IL)\u20101\u03b2 and IL\u20106 in lipopolysaccharide (LPS)\u2010induced RAW 264.7 and HaCaT cells, along with the intracellular reactive oxygen species (ROS) generation. Furthermore, PPH and RPE inhibited the nuclear translocation of nuclear factor kappa\u2010B (NF\u2010\u03baB) P65 in LPS\u2010induced RAW 264.7 cells. Based on molecular docking, PPH could bind to kelch\u2010like ECH\u2010associated protein 1 (KEAP1) protein. PPH and RPE treatments could activate nuclear factor erythroid 2\u2010related factor 2 (NRF2) and upregulate haem oxygenase\u20101 (HO\u20101). Moreover, RPE suppressed the mitogen\u2010activated protein kinase (MAPK) pathway. Therefore, PPH\u2010enriched RPE showed anti\u2010inflammatory and antioxidative effects in\u00a0vitro, which is promising for alternative antiacne therapeutic.<\/jats:p>","DOI":"10.1111\/jcmm.18146","type":"journal-article","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T15:19:35Z","timestamp":1709306375000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Rhizoma Paridis saponins attenuate Gram\u2010negative bacteria\u2010induced inflammatory acne by binding to KEAP1<\/scp> and modulating Nrf2 and MAPK<\/scp> pathways"],"prefix":"10.1111","volume":"28","author":[{"given":"Yang","family":"Yang","sequence":"first","affiliation":[{"name":"R&D Department Yunnan Baiyao Group Health Products Co., Ltd Kunming Yunnan China"},{"name":"R&D Department East Asia Skin Health Research Center Beijing China"},{"name":"R&D Department Yunnan Baiyao Group Shanghai Science & Technology Co., Ltd Shanghai 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