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Recent studies have highlighted the essential role of long noncoding RNAs (lncRNAs) in AD by acting as competing endogenous RNAs (ceRNAs). Our aim was to construct lncRNA\u2010associated ceRNA regulatory networks composed of potential biomarkers for the early stage of AD. AD related datasets come from AlzData and GEO databases. The R package \u2018Limma\u2019 identifies differentially expressed genes (DEGs), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases for functional enrichment analysis. Protein\u2013protein interactions (PPIs) in DEGs were constructed in the STRING database, and Cytoscape software identified DEGs. Convergent functional genomics (CFG) analysis of differentially expressed hub genes (referred to as early\u2010DEGs) in the brain before the development of AD pathology. The AlzData database analyses the expression levels of early\u2010DEGs in different nerve cells. The lncRNA\u2010miRNA\u2010mRNA regulatory network was established according to the ceRNA hypothesis. We identified four lncRNAs (XIST, NEAT1, KCNQ1OT1 and HCG18) and four miRNAs (hsa\u2010let\u20107c\u20105p, hsa\u2010miR\u2010107, hsa\u2010miR\u2010129\u20102\u20103p and hsa\u2010miR\u2010214\u20103p) were preliminarily identified as potential biomarkers for early AD, competitively regulating <jats:italic>Atp6v0b<\/jats:italic>, <jats:italic>Atp6v1e1 Atp6v1f<\/jats:italic> and <jats:italic>Syt1<\/jats:italic>. This study indicates that NEAT1, XIST, HCG18 and KCNQ1OT1 act as ceRNAs in competitive binding with miRNAs to regulate the expression of <jats:italic>Atp6v0b<\/jats:italic>, <jats:italic>Atp6v1e1<\/jats:italic>, <jats:italic>Atp6v1f<\/jats:italic> and <jats:italic>Syt1<\/jats:italic> before the occurrence of pathological changes in AD.<\/jats:p>","DOI":"10.1111\/jcmm.18151","type":"journal-article","created":{"date-parts":[[2024,3,2]],"date-time":"2024-03-02T05:50:36Z","timestamp":1709358636000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Identification of key regulatory molecules in the early development stage of Alzheimer's disease"],"prefix":"10.1111","volume":"28","author":[{"given":"Bin","family":"Huang","sequence":"first","affiliation":[{"name":"Clinical Laboratory Fifth Affiliated Hospital of Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2761-522X","authenticated-orcid":false,"given":"Guan\u2010yong","family":"Ou","sequence":"additional","affiliation":[{"name":"School of Medicine Southern University of Science and Technology  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7973-6966","authenticated-orcid":false,"given":"Ni","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology Shantou University Medical College  Shantou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2015.56"},{"key":"e_1_2_10_3_1","unstructured":"PrinceM WimoA GuerchetM AliGC WuYT PrinaM.World Alzheimer Report 2015. 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