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Calycosin (CA) is a compound with anti\u2010inflammatory and osteoprotective properties. This study aimed to evaluate the effects of CA on titanium (Ti) particle\u2010induced osteolysis, Ti particle\u2010induced macrophage polarization and subsequent osteogenic deficits, and explore the associated signalling pathways in a Ti particle\u2010stimulated calvarial osteolysis mouse model using micro\u2010CT, ELISA, qRT\u2010PCR, immunofluorescence and western blot techniques. The results showed that CA alleviated inflammation, osteogenic inhibition and osteolysis in the Ti particle\u2010induced calvarial osteolysis mouse model in\u00a0vivo. In\u00a0vitro experiments showed that CA suppressed Ti\u2010induced M1 macrophage polarization, promoted M2 macrophage polarization and ultimately enhanced osteogenic differentiation of MC3T3\u2010E1 cells. In addition, CA alleviated osteogenic deficits by regulating macrophage polarization homeostasis via the NF\u2010\u03baB signalling pathway both in\u00a0vivo and in\u00a0vitro. All these findings suggest that CA may prove to be an effective therapeutic agent for wear particle\u2010induced osteolysis.<\/jats:p>","DOI":"10.1111\/jcmm.18157","type":"journal-article","created":{"date-parts":[[2024,3,18]],"date-time":"2024-03-18T02:50:55Z","timestamp":1710730255000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Calycosin alleviates titanium particle\u2010induced osteolysis by modulating macrophage polarization and subsequent osteogenic differentiation"],"prefix":"10.1111","volume":"28","author":[{"given":"Hui","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Orthopedics The Affiliated Jinling Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Affiliated Jinling Hospital of Nanjing Medical University  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