{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T07:45:41Z","timestamp":1782546341114,"version":"3.54.5"},"reference-count":67,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Liver cirrhosis is a silent disease in humans and is experimentally induced by many drugs and toxins as thioacetamide (TAA) in particular, which is the typical model for experimental induction of hepatic fibrosis. Thus, the objective of the present study was to elucidate the possible protective effects of lact\u00e9ol\u00ae forte (LF) and quercetin dihydrate (QD) against TAA\u2010induced hepatic damage in male albino rats. Induction of hepatotoxicity was performed by TAA injection (200\u2009mg\/kg I\/P, twice\/ week) in rats. LF (1\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\u2009CFU\/rat 5 times\/week) and QD (50\u2009mg\/kg 5 times\/week) treated groups were administered concurrently with TAA injection (200\u2009mg\/kg I\/P, twice\/ week). The experimental treatments were conducted for 12\u2009weeks. Hepatotoxicity was evaluated biochemically by measuring alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma\u2010glutamyl transferase (GGT) in the serum and histopathologically with the scoring of histopathological changes besides histochemical assessment of collagen by Masson's trichrome and immunohistochemical analysis for \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA), Ki67 and caspase\u20103 expression in liver sections. Our results indicated that LF and QD attenuated some biochemical changes and histochemical markers in TAA\u2010mediated hepatotoxicity in rats by amelioration of biochemical markers and collagen, \u03b1\u2010SMA, Ki67 and caspase3 Immunoexpression. Additionally, LF and QD supplementation downregulated the proliferative, necrotic, fibroblastic changes, eosinophilic intranuclear inclusions, hyaline globules and Mallory\u2010like bodies that were detected histopathologically in the TAA group. In conclusion, LF showed better hepatic protection than QD against TAA\u2010induced hepatotoxicity in rats by inhibiting inflammatory reactions with the improvement of some serum hepatic transaminases, histopathological picture and immunohistochemical markers.<\/jats:p>","DOI":"10.1111\/jcmm.18196","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T13:34:13Z","timestamp":1711546453000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Hepatoprotective activity of Lact\u00e9ol\u00ae forte and quercetin dihydrate against thioacetamide\u2010induced hepatic cirrhosis in male albino rats"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9555-7300","authenticated-orcid":false,"given":"Hebatallah M.","family":"Saad","sequence":"first","affiliation":[{"name":"Department of Pathology, Faculty of Veterinary Medicine Matrouh University  Matrouh Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samah S.","family":"Oda","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Veterinary Medicine Alexandria University  Abees Alexandria Province Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2206-7236","authenticated-orcid":false,"given":"Athanasios","family":"Alexiou","sequence":"additional","affiliation":[{"name":"University Centre for Research &amp; Development Chandigarh University  Mohali Punjab India"},{"name":"Department of Research &amp; Development Funogen  Athens Greece"},{"name":"Department of Research &amp; Development AFNP Med  Wien Austria"},{"name":"Department of Science and Engineering Novel Global Community Educational Foundation  Hebersham New South Wales Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marios","family":"Papadakis","sequence":"additional","affiliation":[{"name":"Department of Surgery II University Hospital Witten\u2010Herdecke, Heusnerstrasse 40, University of Witten\u2010Herdecke  Wuppertal Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed H.","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Science King Saud University  Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaber El\u2010Saber","family":"Batiha","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine Damanhour University  Damanhour AlBeheira Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman","family":"Khalifa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine Matrouh University  Matrouh Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.05.011"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-022-19383-9"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(02)00011-9"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/715130"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinre.2021.101834"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-021-00499-1"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2015.09.018"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11224-022-01933-z"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.15419\/bmrat.v9i6.745"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.30296"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b00944"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.02.057"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.108653"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.26873\/SVR\u2010793\u20102019"},{"key":"e_1_2_11_16_1","volume-title":"Bancroft's Theory and Practice of Histological Techniques E\u2010Book","author":"Suvarna KS","year":"2018"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.3629"},{"key":"e_1_2_11_18_1","volume-title":"Diagnostic Immunohistochemistry E\u2010Book: Theranostic and Genomic Applications","author":"Dabbs DJ","year":"2021"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.3465"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/69.2.448"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2021.105933"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2021.09.023"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/79.5.727"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10024-003-5061-7"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-003-0397-3"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.08.060"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)01355-7"},{"issue":"5","key":"e_1_2_11_28_1","first-page":"219","article-title":"Pharmacokinetics and bioavailability of the flavonol quercetin in humans","volume":"37","author":"Graefe EU","year":"1999","journal-title":"Int J Clin Pharmacol Ther"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2016.01.029"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/md17060317"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2008.91"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116933"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10060911"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27196470"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1FO04292K"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0260130"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.34172\/jhp.2023.21"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1177\/09603271211026729"},{"issue":"8","key":"e_1_2_11_39_1","first-page":"956","article-title":"The impact of lactobacillus acidophilus on hepatic and colonic fibrosis induced by ethephon in a rat model","volume":"22","author":"Bahr HI","year":"2019","journal-title":"Iran J Basic Med Sci"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp-2017-0651"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-016-9522-9"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12265-020-09997-0"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2006.02.006"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx3002719"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-012-9434-6"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01376"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i23.7312"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2020.04.044"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-7020-5318-4.00008-5"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2017.03.029"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00072"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.2914"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1097-4652(200003)182:3<311::aid-jcp1>3.0.co;2-9"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2016.03.005"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2008.03191.x"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2014.15.1.51"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1177\/0394632015572083"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-7885-x"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines8020329"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.41686"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v12.i32.5175"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2018.01.021"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19082212"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.94"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051764"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200700393"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-374228-5.00027-5"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M305063200"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.18196","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,27]],"date-time":"2024-04-27T04:46:06Z","timestamp":1714193166000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.18196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,27]]},"references-count":67,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1111\/jcmm.18196"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.18196","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,27]]},"assertion":[{"value":"2023-07-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e18196"}}