{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T10:27:46Z","timestamp":1789122466733,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T00:00:00Z","timestamp":1709683200000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study aimed to explore the impact of SCD Probiotics supplementation on biomolecule profiles and histopathology of ileum and colon tissues during a 30\u2010day intermittent fasting (IF) program. Male Sprague\u2013Dawley rats, aged 24\u2009months, underwent 18\u2010h daily fasting and received 3\u2009mL (1\u2009\u00d7\u2009108\u2009CFU) of SCD Probiotics. The differences in biomolecule profiles were determined using FTIR Spectroscopy and two machine learning techniques, Linear Discriminant Analysis (LDA) and Support Vector Machine (SVM), which showed significant differences with high accuracy rates. Spectrochemical bands indicating alterations in lipid, protein and nucleic acid profiles in both tissues. The most notable changes were observed in the group subjected to both IF and SCD Probiotics, particularly in the colon. Both interventions, individually and in combination, decreased protein carbonylation levels. SCD Probiotics exerted a more substantial impact on membrane dynamics than IF alone. Additionally, both IF and SCD Probiotics were found to have protective effects on intestinal structure and stability by reducing mast cell density and levels of TNF\u2010\u03b1 and NF\u2010\u03baB expression in ileum and colon tissues, thus potentially mitigating age\u2010related intestinal damage and inflammation. Furthermore, our results illustrated that while IF and SCD Probiotics individually instigate unique changes in ileum and colon tissues, their combined application yielded more substantial benefits. This study provides evidence for the synergistic potential of IF and SCD Probiotics in combating age\u2010related intestinal alterations.<\/jats:p>","DOI":"10.1111\/jcmm.18203","type":"journal-article","created":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T06:19:39Z","timestamp":1709705979000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Supplementing probiotics during intermittent fasting proves more effective in restoring ileum and colon tissues in aged rats"],"prefix":"10.1111","volume":"28","author":[{"given":"Hikmet Taner","family":"Teker","sequence":"first","affiliation":[{"name":"Department of Molecular Biology Ankara Medipol University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3041-6010","authenticated-orcid":false,"given":"Taha","family":"Ceylani","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Mu\u015f Alparslan University  Mu\u015f Turkey"},{"name":"Department of Food Quality Control and Analysis Mu\u015f Alparslan University  Mu\u015f Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seda","family":"Keskin","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Van Yuzuncu Yil University  Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gizem","family":"Samgane","sequence":"additional","affiliation":[{"name":"Department Biotechnology, Institute of Graduate Education Bilecik \u015eeyh Edebali University  Bilecik Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7192-6669","authenticated-orcid":false,"given":"H\u00fcseyin","family":"Allahverdi","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Mu\u015f Alparslan University  Mu\u015f Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eda","family":"Acikgoz","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Van Yuzuncu Yil University  Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5293-6447","authenticated-orcid":false,"given":"Rafig","family":"Gurbanov","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Bilecik \u015eeyh Edebali University  Bilecik Turkey"},{"name":"Central Research Laboratory Bilecik Seyh Edebali University  Bilecik Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000492573"},{"issue":"3","key":"e_1_2_11_3_1","first-page":"185","article-title":"Intermittent fasting: physiological implications on outcomes in mice and men","volume":"35","author":"Lee JH","year":"2020","journal-title":"Phys Ther"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1905136"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2022.114825"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2018.08.004"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu13093166"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10123-022-00272-7"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-112618-043650"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-022-03154-8"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-019-1616-2"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archger.2023.105003"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2018.e00644"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu7063999"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15214557"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jambio\/lxad092"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2014.66"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-020-1341-1"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.5056\/jnm20149"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.110"},{"key":"e_1_2_11_21_1","first-page":"421","volume-title":"Vibrational Spectroscopy in Diagnosis and Screening","author":"Severcan F","year":"2012"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2023.166799"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2022.166473"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3906\/biy-2105-65"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17926"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10522-023-10032-3"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.16395"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.18014"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2020.1734423"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1089\/chi.2018.0040"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1586\/17474124.2015.1092872"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2021.108785"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14676-4"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2020.103938"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-021-07308-4"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/2931580"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-022-00605-x"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1159291"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202050047"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2017.05.022"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21381"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2022.106873"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.9b00134"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.18203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T02:55:17Z","timestamp":1710557717000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.18203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":42,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1111\/jcmm.18203"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.18203","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3]]},"assertion":[{"value":"2023-11-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e18203"}}