{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:39:10Z","timestamp":1789987150261,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,4,13]],"date-time":"2024-04-13T00:00:00Z","timestamp":1712966400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Curcuma longa,<\/jats:italic> best known for its culinary application as the main constituent of curry powder, has shown potential impact on the reproductive system. This study aimed to investigate the efficacy of <jats:italic>Curcuma longa<\/jats:italic> extract (CLE) on Kidney\u2010Yang deficiency mice induced by hydrocortisone and the possible roles in testosterone secretion in Leydig cells. We evaluated male sexual behaviour, reproductive organ weight, testosterone levels, and histological tissue changes in hydrocortisone\u2010induced mice. CLE effectively reversed hydrocortisone\u2010induced Kidney\u2010Yang deficiency syndrome by improving sexual behaviour, testis and epididymis weight, testosterone levels and reducing pathological damage. Our in\u00a0vitro study further indicated that CLE stimulated testosterone production via upregulating the mRNA and protein expression of steroidogenic enzymes in Leydig cells. It significantly improved H89\u2010inhibited protein expression of StAR and cAMP\u2010response element\u2010binding (CREB), as well as melatonin\u2010suppressed StAR protein expression. The data obtained from this study suggest that CLE could alleviate Kidney\u2010Yang deficiency symptoms and stimulate testosterone production by upregulating the steroidogenic pathway. This research identifies CLE as a potential nutraceutical option for addressing testosterone deficiency diseases.<\/jats:p>","DOI":"10.1111\/jcmm.18303","type":"journal-article","created":{"date-parts":[[2024,4,13]],"date-time":"2024-04-13T09:04:56Z","timestamp":1712999096000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of <i>Curcuma longa<\/i> extract on reproduction function in mice and testosterone production in Leydig cells"],"prefix":"10.1111","volume":"28","author":[{"given":"Yisheng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pharmacy Wuhan Hospital of Traditional Chinese Medicine  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Wuhan Hospital of Traditional Chinese Medicine  Wuhan Hubei China"},{"name":"Hubei Province Key Laboratory of Traditional Chinese Medicine Resource and Chemistry, Hubei Shizhen Laboratory, Hubei University of Chinese Medicine  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Xue","sequence":"additional","affiliation":[{"name":"Hubei Province Key Laboratory of Traditional Chinese Medicine Resource and Chemistry, Hubei Shizhen Laboratory, Hubei University of Chinese Medicine  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hubei Province Key Laboratory of Traditional Chinese Medicine Resource and Chemistry, Hubei Shizhen Laboratory, Hubei University of Chinese Medicine  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihan","family":"Li","sequence":"additional","affiliation":[{"name":"Hubei Province Key Laboratory of Traditional Chinese Medicine Resource and Chemistry, Hubei Shizhen Laboratory, Hubei University of Chinese Medicine  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hubei Province Key Laboratory of Traditional Chinese Medicine Resource and Chemistry, Hubei Shizhen Laboratory, Hubei University of Chinese Medicine  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoyin","family":"Kai","sequence":"additional","affiliation":[{"name":"College of pharmacy Zhejiang Chinese Medical University  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9838-0990","authenticated-orcid":false,"given":"Juan","family":"Li","sequence":"additional","affiliation":[{"name":"Hubei Province Key Laboratory of Traditional Chinese Medicine Resource and Chemistry, Hubei Shizhen Laboratory, Hubei University of Chinese Medicine  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2019.108729"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7656919"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2018.09.035"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevEukaryotGeneExpr.2018020088"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13197-019-03986-1"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6324"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118051"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.contraception.2009.01.001"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13952"},{"key":"e_1_2_10_11_1","first-page":"71","article-title":"Contraceptive effect of Curcuma longa (L.) in male albino rat","volume":"6","author":"Ashok P","year":"2004","journal-title":"Asian J Androl"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119575"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ame2.12081"},{"key":"e_1_2_10_14_1","first-page":"190","article-title":"Study the protective effect of Curcuma Longa rhizomes extract against the toxicity of lead acetate that induce infertility in male albino rats","volume":"22","author":"Hussein RS","year":"2017","journal-title":"Tikrit Medical Journal"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.01.072"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.13641"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/10408444.2016.1140718"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2010.09.026"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-18065-3"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr300965d"},{"key":"e_1_2_10_21_1","first-page":"427","article-title":"Techniques and basic experiments for the study of brain and behavior","volume":"2","author":"Bure\u0161 JBOH","year":"1984","journal-title":"J Neurol Sci"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(70)90050-8"},{"issue":"341","key":"e_1_2_10_23_1","first-page":"344","article-title":"Isolation of Sertoli, Leydig, and spermatogenic cells from the mouse testis","volume":"51","author":"Chang YF","year":"2011","journal-title":"BioTechniques"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02913063"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.3.6585"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-080-5_20"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.122re4"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2012.04.031"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hermed.2015.03.001"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389200220666191001104834"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.EJ18-0010"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(00)00056-1"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2015.08.001"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-1046"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02142-w"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2001.tb02177.x"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2015.07.009"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.18303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,27]],"date-time":"2024-04-27T04:45:18Z","timestamp":1714193118000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.18303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":37,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1111\/jcmm.18303"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.18303","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"2023-11-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e18303"}}