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However, the exact role of miR\u2010429\u2010CRKL axis in leukaemic cell differentiation are still unknown. The current work aimed to uncover the effect of miR\u2010429\u2010CRKL axis on erythropoiesis. In the present study, CRKL upregulation was negatively correlated with miR\u2010429 downregulation in both chronic myeloid leukaemia (CML) patient and CR patient samples. Moreover, CRKL expression level was significantly decreased while miR\u2010429 expression level was increased during the erythroid differentiation of K562 cells following hemin treatment. Functional investigations revealed that overexpression and knockdown of CRKL was remarkably effective in suppressing and promoting hemin\u2010induced erythroid differentiation of K562 cells, whereas, miR\u2010429 exhibited opposite effects to CRKL. Mechanistically, miR\u2010429 regulates erythroid differentiation of K562 cells by downregulating CRKL via selectively targeting <jats:italic>CRKL<\/jats:italic>\u20103\u2032\u2010untranslated region (UTR) through Raf\/MEK\/ERK pathway. Conversely, CRKII had no effect on erythroid differentiation of K562 cells. Taken together, our data demonstrated that CRKL (but not CRKII) and miR\u2010429 contribute to development, progression and erythropoiesis of CML, miR\u2010429\u2010CRKL axis regulates erythropoiesis of K562 cells via Raf\/MEK\/ERK pathway, providing novel insights into effective diagnosis and therapy for CML patients.<\/jats:p>","DOI":"10.1111\/jcmm.18308","type":"journal-article","created":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T13:59:55Z","timestamp":1714399195000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>CRKL<\/scp> but not <scp>CRKII<\/scp> contributes to hemin\u2010induced erythroid differentiation of <scp>CML<\/scp>"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8765-0054","authenticated-orcid":false,"given":"Chunmei","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Biotechnology &amp; Liaoning Key Laboratory of Cancer Stem 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