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However, the mechanisms underlying gallbladder carcinogenesis and its progression are poorly understood. The function of m6A modification and its regulators was still unclear for gallbladder cancer. The current study seeks to investigate the function of YTH m6A RNA\u2010binding protein 1 (YTHDF1) in gallbladder cancer. Transcriptomic analysis and immunochemical staining of YTHDF1 in gallbladder cancer tissues revealed its upregulation compared to paracancerous tissues. Moreover, YTHDF1 promotes the proliferation assays, Transwell migration assays, and Transwell invasion assays of gallbladder cancer cells in\u00a0vitro. And it also increased tumour growth in xenograft mouse model and metastases in tail vein injection model in\u00a0vivo. In\u00a0vitro, UHRF1 knockdown partly reversed the effects of YTHDF1 overexpression. Mechanistically, dual\u2010luciferase assays proved that YTHDF1 promotes <jats:italic>UHRF1<\/jats:italic> expression via direct binding to the mRNA 3\u2032\u2010UTR in a m6A\u2010dependent manner. Overexpression of YTHDF1 enhanced UHRF1 mRNA stability, as demonstrated by mRNA stability assays, and Co\u2010IP studies confirmed a direct interaction between YTHDF1 and PABPC1. Collectively, these findings provide new insights into the progression of gallbladder cancer as well as a novel post\u2010transcriptional mechanism of YTHDF1 via stabilizing target mRNA.<\/jats:p>","DOI":"10.1111\/jcmm.18328","type":"journal-article","created":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T13:59:50Z","timestamp":1714399190000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>YTHDF1<\/scp> promotes gallbladder cancer progression via post\u2010transcriptional regulation of the <scp>m6A<\/scp>\/<scp>UHRF1<\/scp> axis"],"prefix":"10.1111","volume":"28","author":[{"given":"Jiemin","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Complex Severe and Rare Diseases, Department of Gastroenterology Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences  Beijing 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