{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:55:12Z","timestamp":1791532512677,"version":"4.3.4"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T00:00:00Z","timestamp":1714435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007847","name":"Natural Science Foundation of Jilin Province","doi-asserted-by":"publisher","award":["20200201595JC"],"award-info":[{"award-number":["20200201595JC"]}],"id":[{"id":"10.13039\/100007847","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010211","name":"Education Department of Jilin Province","doi-asserted-by":"publisher","award":["JJKH20231220KJ"],"award-info":[{"award-number":["JJKH20231220KJ"]}],"id":[{"id":"10.13039\/501100010211","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Infections caused by <jats:italic>Candida<\/jats:italic> species, especially <jats:italic>Candida albicans<\/jats:italic>, threaten the public health and create economic burden. Shortage of antifungals and emergence of drug resistance call for new antifungal therapies while natural products were attractive sources for developing new drugs. In our study, fangchinoline, a bis\u2010benzylisoquinoline alkaloid from Chinese herb <jats:italic>Stephania tetrandra<\/jats:italic> S. Moore, exerted antifungal effects on planktonic growth of several <jats:italic>Candida<\/jats:italic> species including <jats:italic>C.\u2009albicans<\/jats:italic>, with MIC no more than 50\u2009\u03bcg\/mL. In addition, results from microscopic, MTT and XTT reduction assays showed that fangchinoline had inhibitory activities against the multiple virulence factors of <jats:italic>C.\u2009albicans<\/jats:italic>, such as adhesion, hyphal growth and biofilm formation. Furthermore, this compound could also suppress the metabolic activity of preformed <jats:italic>C.\u2009albicans<\/jats:italic> biofilms. PI staining, followed by confocal laser scanning microscope (CLSM) analysis showed that fangchinoline can elevate permeability of cell membrane. DCFH\u2010DA staining suggested its anti\u2010<jats:italic>Candida<\/jats:italic> mechanism also involved overproduction of intracellular ROS, which was further confirmed by N\u2010acetyl\u2010cysteine rescue tests. Moreover, fangchinoline showed synergy with three antifungal drugs (amphotericin B, fluconazole and caspofungin), further indicating its potential use in treating <jats:italic>C.\u2009albicans<\/jats:italic> infections. Therefore, these results indicated that fangchinoline could be a potential candidate for developing anti\u2010<jats:italic>Candida<\/jats:italic> therapies.<\/jats:p>","DOI":"10.1111\/jcmm.18354","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T08:44:08Z","timestamp":1714466648000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Fangchinoline inhibits growth and biofilm of <i>Candida albicans<\/i> by inducing <scp>ROS<\/scp> overproduction"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4153-9670","authenticated-orcid":false,"given":"Longfei","family":"Yang","sequence":"first","affiliation":[{"name":"Jilin Provincial Key Laboratory on Molecular and Chemical Genetics The Second Hospital of Jilin University  Changchun 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