{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T13:58:21Z","timestamp":1787579901672,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T00:00:00Z","timestamp":1714089600000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102022R1F1A106940312"],"award-info":[{"award-number":["NRF\u20102022R1F1A106940312"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102023\u201000217595"],"award-info":[{"award-number":["RS\u20102023\u201000217595"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Trichospira verticillata<\/jats:italic> is an annual herb that belongs to the family <jats:italic>Asteraceae<\/jats:italic>. <jats:italic>Trichospira verticillata<\/jats:italic> extract (TVE) elicits anti\u2010plasmodial activity; however, there has been no detailed report about its anti\u2010inflammatory effects and molecular mechanisms. In addition, herbal plants exhibit anti\u2010inflammatory effects by suppressing the NLRP3 inflammasome. Therefore, the primary goal of this study was to examine the effects of TVE on NLRP3 inflammasome activation by measuring interleukin\u20101\u03b2 (IL\u20101\u03b2) secretion. We treated lipopolysaccharides (LPS)\u2010primed J774A.1 and THP\u20101 cells with TVE, which attenuated NLRP3 inflammasome activation. Notably, TVE did not affect nuclear factor\u2010kappa B (NF\u2010\u03baB) signalling or intracellular reactive oxygen species (ROS) production and potassium efflux, suggesting that it inactivates the NLRP3 inflammasome via other mechanisms. Moreover, TVE suppressed the formation of apoptosis\u2010associated speck\u2010like protein (ASC) speck and oligomerization. Immunoprecipitation data revealed that TVE reduced the binding of NLRP3 to NIMA\u2010related kinase 7 (NEK7), resulting in reduced ASC oligomerization and speck formation. Moreover, TVE alleviated neutrophilic asthma (NA) symptoms in mice. This study demonstrates that TVE modulates the binding of NLPR3 to NEK7, thereby reporting novel insights into the mechanism by which TVE inhibits NLRP3 inflammasome. These findings suggest TVE as a potential therapeutic of NLRP3 inflammasome\u2010mediated diseases, particularly NA.<\/jats:p>","DOI":"10.1111\/jcmm.18356","type":"journal-article","created":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T15:04:01Z","timestamp":1714143841000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Anti\u2010inflammatory effect of <i>Trichospira verticillata<\/i> via suppression of the <scp>NLRP3<\/scp> inflammasome in neutrophilic asthma"],"prefix":"10.1111","volume":"28","author":[{"given":"Hyeyun","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Microbiology Ajou University School of Medicine  Suwon Republic of Korea"},{"name":"Department of Biomedical Sciences Graduate School of Ajou University  Suwon Republic of 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