{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:16:52Z","timestamp":1788779812276,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012543","name":"Youth Science Foundation of Guangxi Medical University","doi-asserted-by":"publisher","award":["GXMUYSF202420"],"award-info":[{"award-number":["GXMUYSF202420"]}],"id":[{"id":"10.13039\/501100012543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Increasing evidence has shown that homologous recombination (HR) and metabolic reprogramming are essential for cellular homeostasis. These two processes are independent as well as closely intertwined. Nevertheless, they have rarely been reported in lung adenocarcinoma (LUAD). We analysed the genomic, immune microenvironment and metabolic microenvironment features under different HR activity states. Using cell cycle, EDU and cell invasion assays, we determined the impacts of si\u2010SHFM1 on the LUAD cell cycle, proliferation and invasion. The levels of isocitrate dehydrogenase (IDH) and \u03b1\u2010ketoglutarate dehydrogenase (\u03b1\u2010KGDH) were determined by ELISA in the NC and si\u2010SHFM1 groups of A549 cells. Finally, cell samples were used to extract metabolites for HPIC\u2010MS\/MS to analyse central carbon metabolism. We found that high HR activity was associated with a poor prognosis in LUAD, and HR was an independent prognostic factor for TCGA\u2010LUAD patients. Moreover, LUAD samples with a high HR activity presented low immune infiltration levels, a high degree of genomic instability, a good response status to immune checkpoint blockade therapy and a high degree of drug sensitivity. The si\u2010SHFM1 group presented a significantly higher proportion of cells in the G0\/G1 phase, lower levels of DNA replication, and significantly lower levels of cell migration and both TCA enzymes. Our current results indicated that there is a strong correlation between HR and the TCA cycle in LUAD. The TCA cycle can promote SHFM1\u2010mediated HR in LUAD, raising their activities, which can finally result in a poor prognosis and impair immunotherapeutic efficacy.<\/jats:p>","DOI":"10.1111\/jcmm.18406","type":"journal-article","created":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T04:07:13Z","timestamp":1717214833000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Metabolic reprogramming\u2010driven homologous recombination and TCA cycle dysregulation contribute to poor prognoses in lung adenocarcinoma"],"prefix":"10.1111","volume":"28","author":[{"given":"Zhanyu","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongming","family":"He","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuliu","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqi","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwen","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiaozhan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8187-3676","authenticated-orcid":false,"given":"Shikang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,31]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-19-0221"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-015-0114-x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201701026"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.03.082"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-17-0492"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.564601"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/onco.13829"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djy085"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12040862"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1140958"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2019.05.023"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw5473"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2018.06.005"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00784"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-7"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.03.077"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3612"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-1070-5"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-020-0721-z"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2022-005226"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdy495"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-20-0522"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-019-09668-y"},{"key":"e_1_2_10_27_1","first-page":"1181","article-title":"Caveolin\u20101\u2010ACE2 axis modulates xenobiotic metabolism\u2010linked chemoresistance in ovarian clear cell carcinoma","volume":"1","author":"Nagappan A","year":"2022","journal-title":"Cell Biol Toxicol"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-022-02315-3"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1062-1"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00412-017-0658-1"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.TM118.000372"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18325"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djv437"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.311"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-12-1170"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neo.2015.09.006"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13184501"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.11.015"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.103366"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201911217"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg090"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-013-1385-5"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2008.08.044"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01229-06"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-16-0457"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgs279"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-13-562"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.18406","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T07:12:05Z","timestamp":1719213125000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.18406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,31]]},"references-count":46,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1111\/jcmm.18406"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.18406","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,31]]},"assertion":[{"value":"2024-01-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e18406"}}