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Since no approved antiviral drugs nor licensed active vaccines are yet available, we aimed to utilize immunoinformatics approach to design chimeric vaccine against the two mentioned viruses. This is the first study to deal with design divalent vaccine against SARS\u2010CoV\u20102 and MPXV. ORF8, E and M proteins from Omicron SARS\u2010CoV\u20102 and gp182 from MPXV were used as the protein precursor from which multi\u2010epitopes (inducing B\u2010cell, helper T cells, cytotoxic T cells and interferon\u2010\u0263) chimeric vaccine was contrived. The structure of the vaccine construct was predicted, validated, and docked to toll\u2010like receptor\u20102 (TLR\u20102). Moreover, its sequence was also used to examine the immune simulation profile and was then inserted into the pET\u201028a plasmid for in silico cloning. The vaccine construct was probable antigen (0.543) and safe (non\u2010allergen) with strong binding energy to TLR\u20102 (\u22121169.8\u2009kcal\/mol) and found to have significant immune simulation profile. In conclusion, the designed chimeric vaccine was potent and safe against SARS\u2010CoV\u20102 and MPXV, which deserves further consideration.<\/jats:p>","DOI":"10.1111\/jcmm.18452","type":"journal-article","created":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T14:38:48Z","timestamp":1716820728000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Design of multi\u2010epitope chimeric vaccine against Monkeypox virus and SARS\u2010CoV\u20102: A vaccinomics perspective"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3850-244X","authenticated-orcid":false,"given":"Haitham","family":"Al\u2010Madhagi","sequence":"first","affiliation":[{"name":"Biochemical Technology Program, Faculty of Applied Sciences Dhamar University  Dhamar Yemen"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adeela","family":"Kanawati","sequence":"additional","affiliation":[{"name":"Division of Biochemistry, Chemistry Department University of Aleppo  Aleppo Syria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zaher","family":"Tahan","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Biology Department University of Aleppo  Aleppo Syria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,27]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"CDC.Monkeypox outbreak global map.https:\/\/www.cdc.gov\/poxvirus\/monkeypox\/response\/2022\/world\u2010map.html2022."},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010141"},{"key":"e_1_2_10_4_1","first-page":"6","article-title":"Emergence of monkeypox as the most important orthopoxvirus infection in humans. 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