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This study used differential analysis and weighted co\u2010expression network analysis (WGCNA) to identify susceptibility modules and hub genes associated with fracture healing. Two datasets, GSE125289 and GSE213891, were downloaded from the GEO website, and differentially expressed miRNAs and genes were analysed and used to construct the WGCNA network. Gene ontology (GO) analysis of the differentially expressed genes showed enrichment in cytokine and inflammatory factor secretion, phagocytosis, and trans\u2010Golgi network regulation pathways. Using bioinformatic site prediction and crossover gene search, miR\u201029b\u20103p was identified as a regulator of LIN7A expression that may negatively affect fracture healing. Potential miRNA\u2010mRNA interactions in the bone non\u2010union mechanism were explored, and miRNA\u201029\u20103p and LIN7A were identified as biomarkers of skeletal non\u2010union. The expression of miRNA\u201029b\u20103p and LIN7A was verified in blood samples from patients with fracture non\u2010union using qRT\u2010PCR and ELISA. Overall, this study identified characteristic modules and key genes associated with fracture non\u2010union and provided insight into its molecular mechanisms. Downregulated miRNA\u201029b\u20103p was found to downregulate LIN7A protein expression, which may affect the healing process after fracture in patients with bone non\u2010union. These findings may serve as a prognostic biomarker and potential therapeutic target for bone non\u2010union.<\/jats:p>","DOI":"10.1111\/jcmm.18522","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T07:14:11Z","timestamp":1719990851000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification and validation of <scp>miR<\/scp>\u201029b\u20103p and <scp>LIN7A<\/scp> as important diagnostic markers for bone non\u2010union by <scp>WGCNA<\/scp>"],"prefix":"10.1111","volume":"28","author":[{"given":"Guojian","family":"Jian","sequence":"first","affiliation":[{"name":"Department of Orthopedics Chenggong Hospital of Xiamen University (the 73th Group Military Hospital of People's Liberation Army)  Xiamen Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Desheng","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Orthopedics 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