{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:10:28Z","timestamp":1789578628162,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:00:00Z","timestamp":1721865600000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2501700"],"award-info":[{"award-number":["2021YFC2501700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2501705"],"award-info":[{"award-number":["2021YFC2501705"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Despite numerous investigations on the influence of fibroblast growth factor 23 (FGF23), \u03b1\u2010Klotho and FGF receptor\u20101 (FGFR1) on osteoporosis (OP), there is no clear consensus. Mendelian randomization (MR) analysis was conducted on genome\u2010wide association studies (GWASs)\u2010based datasets to evaluate the causal relationship between FGF23, \u03b1\u2010Klotho, FGFR1 and OP. The primary endpoint was the odds ratio (OR) of the inverse\u2010variance weighted (IVW) approach. Furthermore, we stably transfected FGF23\u2010mimic or siRNA\u2010FGF23 into human bone marrow mesenchymal stem cells (hBMSCs) in culture and determined its cell proliferation and the effects on osteogenic differentiation. Using MR analysis, we demonstrated a strong correlation between serum FGF23 levels and Heel\u2010 and femoral neck\u2010BMDs, with subsequent ORs of 0.919 (95% CI: 0.860\u20130.983,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.014) and 0.751 (95% CI: 0.587\u20130.962;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.023), respectively. The expression levels of FGF23 were significantly increased in femoral neck of patients with OP than in the control cohort (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). Based on our in\u00a0vitro investigation, after overexpression of FGF23, compared to the control group, the BMSC's proliferation ability decreased, the expression level of key osteogenic differentiation genes (RUNX2, OCN and OSX) significantly reduced, mineralized nodules and ALP activity significantly decreased. After silencing FGF23, it showed a completely opposite trend. Augmented FGF23 levels are causally associated with increased risk of OP. Similarly, FGF23 overexpression strongly inhibits the osteogenic differentiation of hBMSCs, thereby potentially aggravating the pathological process of OP.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.18551","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T23:56:04Z","timestamp":1721951764000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Fibroblast growth factor 23\u2010mediated regulation of osteoporosis: Assessed via Mendelian randomization and in\u00a0vitro study"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6465-9483","authenticated-orcid":false,"given":"Xiang","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Endocrine Glucose &amp; Lipids Metabolism and Brain Aging, Ministry of Education; Department of Endocrinology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"},{"name":"Shandong Key Laboratory of Endocrinology and Lipid Metabolism  Jinan Shandong China"},{"name":"Shandong Institute of Endocrine and Metabolic Diseases  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6557-3859","authenticated-orcid":false,"given":"Jin","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Endocrine Glucose &amp; Lipids Metabolism and Brain Aging, Ministry of Education; Department of Endocrinology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"},{"name":"Shandong Key Laboratory of Endocrinology and Lipid Metabolism  Jinan Shandong China"},{"name":"Shandong Institute of Endocrine and Metabolic Diseases  Jinan Shandong China"},{"name":"\u201cChuangxin China\u201d Innovation Base of stem cell and Gene Therapy for endocrine Metabolic diseases  Jinan Shandong China"},{"name":"Shandong Engineering Laboratory of Prevention and Control for Endocrine and Metabolic Diseases  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-022-06454-3"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005010052"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-25040-4"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2006.05.002"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semnephrol.2014.09.005"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2016010098"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/sim.3034"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2018020192"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a115184"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-003-1560-7"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.060110"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwf113"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-002-2159-2"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/1047-2797(91)90005-W"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv402"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1517\/14622416.2.1.S1"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dys066"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dys064"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.06.110"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abj1541"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/sim.6835"},{"issue":"6","key":"e_1_2_11_23_1","first-page":"1961","article-title":"Assessing the suitability of summary data for two\u2010sample Mendelian randomization analyses using MR\u2010Egger regression: the role of the I2 statistic","volume":"45","author":"Bowden J","year":"2016","journal-title":"Int J Epidemiol"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/gepi.21965"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dyt179"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10654-017-0255-x"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/gepi.22207"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2012.05.001"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.braindev.2019.12.010"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21145169"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc13-2235"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.263"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/7536614"},{"issue":"7","key":"e_1_2_11_34_1","first-page":"945","article-title":"[Hormones and osteoporosis update. 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