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Although glucocorticoids are commonly used in clinical practice, the mechanism by which the active compound Epimedin C (EC) alleviates glucocorticoid\u2010induced osteoporosis (GIOP) is not well understood. The therapeutic potential of EC in treating GIOP was evaluated using alizarin red S staining, calcein immersion and fluorescence imaging, and bone mineralization, bone mass accumulation and bone density in zebrafish larvae were determined. Using the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the key signalling pathways related to bone development were identified. A protein\u2013protein interaction network (PPIN) was constructed to identify osteoclast characteristic genes and the findings were verified using real\u2010time quantitative PCR (RT\u2010qPCR). The bone tissue damage caused by prednisolone was reduced by EC. It also altered physiological processes, improved bone density, boosted mineralization and increased bone mass and activity. Subsequent empirical investigations showed that EC impacted the major signalling pathways involved in bone development, such as osteoclast differentiation, oestrogen, MAPK, insulin resistance, PPAR and AMPK signalling pathways. It also decreased the expression of genes typical of osteoclasts. The results of our study uncover a previously unknown function of EC in controlling bone formation and emphasize the potential of EC as a therapeutic target. The osteoprotective effect of EC indicates its potential as a cost\u2010effective strategy for treating GIOP.<\/jats:p>","DOI":"10.1111\/jcmm.18569","type":"journal-article","created":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T00:39:02Z","timestamp":1722213542000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Functions of Epimedin C in a zebrafish model of glucocorticoid\u2010induced osteoporosis"],"prefix":"10.1111","volume":"28","author":[{"given":"Xiaoyang","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Orthopedics, Xiangyang No. 1 People's Hospital Hubei University of Medicine  Xiangyang China"},{"name":"Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Xiangyang City, Department of Obstetrics and Gynaecology, Xiangyang No. 1 People's Hospital Hubei University of Medicine  Xiangyang 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