{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T06:07:10Z","timestamp":1779430030042,"version":"3.53.1"},"reference-count":36,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:00:00Z","timestamp":1721865600000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Diagnosis of intravascular large B\u2010cell lymphoma (IVLBCL) is a challenge due to its heterogeneous clinical presentation and lack of specific markers. This retrospective study investigated the utility of circulating tumour DNA (ctDNA) sequencing for diagnosing IVLBCL and analysing its mutation landscape. A cohort of 34 IVLBCL patients enrolled and underwent plasma ctDNA targeted sequencing. The median plasma ctDNA concentration was 135.0\u2009ng\/mL, significantly higher than that in diffuse large B\u2010cell lymphoma (DLBCL) controls. Correlations were found between ctDNA concentration and disease severity indicators, LDH and SF. Mutation analysis revealed frequent mutations in B\u2010cell receptor and NF\u2010\u03baB signalling pathways, including\n                    <jats:italic>MYD88<\/jats:italic>\n                    (56%),\n                    <jats:italic>CD79B<\/jats:italic>\n                    (44%),\n                    <jats:italic>TNFAIP3<\/jats:italic>\n                    (38%) and\n                    <jats:italic>IRF4<\/jats:italic>\n                    (29%). CNS involvement was significantly related with BCL6 and CD58 mutation. Patients with complicated hemophagocytic lymphohistiocytosis had significantly higher mutation rates in\n                    <jats:italic>B2M<\/jats:italic>\n                    . Comparison with DLBCL subtypes showed distinctive mutation profiles in IVLBCL. Moreover, plasma ctDNA detected more mutations with higher variant allele fraction than tissue DNA, suggesting its superiority in sensitivity and accessibility. Dynamic monitoring of ctDNA during treatment correlated with therapeutic responses. This study revealed the role of ctDNA in IVLBCL diagnosis, mutation analysis, and treatment monitoring, offering a promising avenue for improving patient diagnosis in this rare lymphoma subtype.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.18576","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T23:56:24Z","timestamp":1721951784000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Plasma circulating tumour\n                    <scp>DNA<\/scp>\n                    is a better source for diagnosis and mutational analysis of\n                    <scp>IVLBCL<\/scp>\n                    than tissue\n                    <scp>DNA<\/scp>"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7939-6241","authenticated-orcid":false,"given":"Chao","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Hematology, Peking Union Medical 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