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However, it is unknown whether <jats:italic>Klf4<\/jats:italic> is involved in acute seizure. To investigate the potential role of <jats:italic>Klf4<\/jats:italic> in pentylenetetrazol (PTZ)\u2010induced seizure, western blotting, immunofluorescence, behaviour test and electrophysiology were conducted in this study. We found that Klf4 protein and mRNA expression were increased in both the hippocampus (HP) and prefrontal cortex (PFC) after PTZ\u2010induced seizure in mice. HP\u2010specific knockout (KO) of <jats:italic>Klf4<\/jats:italic> in mice decreased protein expression of Klf4 and the down\u2010stream <jats:italic>Klf4<\/jats:italic> target tumour protein 53 (TP53\/P53). These molecular changes are accompanied by increased seizure latency, reduced immobility time in the forced swimming test and tail suspension test. Reduced hippocampal protein levels for synaptic proteins, including glutamate receptor 1 (GRIA1\/GLUA1) and postsynaptic density protein 95 (DLG4\/PSD95), were also observed after <jats:italic>Klf4<\/jats:italic>\u2010KO, while increased mRNA levels of complement proteins were observed for <jats:italic>complement component 1q subcomponent A (C1qa)<\/jats:italic>, <jats:italic>complement component 1q subcomponent B<\/jats:italic> (<jats:italic>C1qb<\/jats:italic>), <jats:italic>complement component 1q subcomponent C<\/jats:italic> (<jats:italic>C1qc<\/jats:italic>), <jats:italic>complement component 3<\/jats:italic> (<jats:italic>C3<\/jats:italic>), <jats:italic>complement component 4A<\/jats:italic> (<jats:italic>C4a<\/jats:italic>) and <jats:italic>complement component 4B<\/jats:italic> (<jats:italic>C4b<\/jats:italic>). Moreover, c\u2010Fos expression induced by PTZ was reduced by hippocampal conditional <jats:italic>KO<\/jats:italic> of <jats:italic>Klf4<\/jats:italic>. Electrophysiology showed that PTZ\u2010induced action potential frequency was decreased by overexpression of <jats:italic>Klf4<\/jats:italic>. In conclusion, these findings suggest that <jats:italic>Klf4<\/jats:italic> plays an important role in regulating PTZ\u2010induced seizures and therefore constitutes a new molecular target that should be explored for the development of antiepileptic drugs.<\/jats:p>","DOI":"10.1111\/jcmm.18578","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T11:27:03Z","timestamp":1725535623000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of Kruppel\u2010like factor 4 on <scp>PTZ<\/scp>\u2010induced acute seizure mice"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2612-4285","authenticated-orcid":false,"given":"Bingjin","family":"Li","sequence":"first","affiliation":[{"name":"Jilin Provincial Key Laboratory on Molecular and Chemical Genetics Second Hospital of Jilin University  Changchun People's Republic of China"},{"name":"Department of 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