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After astrocyte exosomes were injected into the lateral ventricle, infarct volume, damage to the blood\u2013brain barrier (BBB), apoptosis and the expression of microglia\u2010related proteins were measured. The dual luciferase reporter assay was used to detect the target genes of miR\u2010628, and overexpressing A2\u2010Exos overexpressed and knocked down miR\u2010628 were constructed. qRT\u2013PCR, western blotting and immunofluorescence staining were subsequently performed. A2\u2010Exos obviously reduced the infarct volume, damage to the BBB and apoptosis and promoted M2 microglial polarization. RT\u2013PCR showed that miR\u2010628 was highly expressed in A2\u2010Exos. Dual luciferase reporter assays revealed that NLRP3, S1PR3 and IRF5 are target genes of miR\u2010628. After miR\u2010628 was overexpressed or knocked down, the protective effects of A2\u2010Exos increased or decreased, respectively. A2\u2010Exos reduced pyroptosis and BBB damage and promoted M2 microglial polarization through the inhibition of NLRP3, S1PR3 and IRF5 via the delivery of miR\u2010628. This study explored the mechanism of action of A2\u2010Exos and provided new therapeutic targets and concepts for treating cerebral ischemia.<\/jats:p>","DOI":"10.1111\/jcmm.70004","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T17:38:50Z","timestamp":1724089130000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<scp>A2<\/scp> reactive astrocyte\u2010derived exosomes alleviate cerebral ischemia\u2013reperfusion injury by delivering <scp>miR<\/scp>\u2010628"],"prefix":"10.1111","volume":"28","author":[{"given":"Yingju","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital of Harbin Medical University  Harbin Heilongjiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology The First 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