{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T14:07:08Z","timestamp":1782914828973,"version":"3.54.5"},"reference-count":62,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T00:00:00Z","timestamp":1726704000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82201147"],"award-info":[{"award-number":["82201147"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171020"],"award-info":[{"award-number":["82171020"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to identify feature genes and explore the molecular mechanisms of keratoconus (KC). We downloaded data files from NCBI GEO public database. The Limma package was used for differential expression analysis of gene profiles. Lasso regression was used to identify the feature genes. The CIBERSORT algorithm was used to infer the proportion of immune\u2010infiltrating cells and analyse the correlation between gene expression levels and immune cells. Related transcription factors and miRNAs of key genes were predicted using the Cistrome DB and Mircode databases. Analysis of expression differences in disease genes was based on the GeneCards database. The CMap was used to analyse targeted therapeutic drugs. IHC was performed to verify the expression levels of ATOH7 and MYRF in corneas. Exactly 593 upregulated and 473 downregulated genes were identified. Lasso regression analysis identified <jats:italic>ATOH7<\/jats:italic>, <jats:italic>DBNDD1<\/jats:italic>, <jats:italic>RNF217\u2010AS1<\/jats:italic>, <jats:italic>ARL11<\/jats:italic>, <jats:italic>MYRF<\/jats:italic> and <jats:italic>SNORA74B<\/jats:italic> as feature genes for KC. All key genes were correlated with immune infiltration and the levels of activated memory CD4+ T cells and plasma cells were significantly increased. miRNA, IRF and STAT families were correlated to feature genes. The expression levels of key genes were significantly correlated to KC\u2010related genes. Entinostat, ochratoxin\u2010a, diphencyprone and GSK\u20103\u2010inhibitor\u2010II were predicted as potential KC medications. The expression of MYRF was significantly higher in the KC samples, contrary to the expression of ATOH7. KC is related to both immune infiltration and genetic factors. <jats:italic>MYRF<\/jats:italic> and <jats:italic>ATOH7<\/jats:italic> were newly identified and verified feature genes of KC.<\/jats:p>","DOI":"10.1111\/jcmm.70079","type":"journal-article","created":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T03:51:37Z","timestamp":1726804297000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Multi\u2010dataset identification of innovative feature genes and molecular mechanisms in keratoconus"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3458-0705","authenticated-orcid":false,"given":"Ning","family":"Lyu","sequence":"first","affiliation":[{"name":"Eye Institute and Department of Ophthalmology Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"NHC Key laboratory of Myopia and Related Eye Diseases, Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration Eye &amp; ENT Hospital, Fudan University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqin","family":"Dai","sequence":"additional","affiliation":[{"name":"Eye Institute and Department of Ophthalmology Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"NHC Key laboratory of Myopia and Related Eye Diseases, Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration Eye &amp; ENT Hospital, Fudan University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawen","family":"Wu","sequence":"additional","affiliation":[{"name":"Eye Institute and Department of Ophthalmology Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"NHC Key laboratory of Myopia and Related Eye Diseases, Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration Eye &amp; ENT Hospital, Fudan University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yidan","family":"Fan","sequence":"additional","affiliation":[{"name":"Eye Institute and Department of Ophthalmology Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"NHC Key laboratory of Myopia and Related Eye Diseases, Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration Eye &amp; ENT Hospital, Fudan University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyuan","family":"Lyu","sequence":"additional","affiliation":[{"name":"Graduate School of Transdisciplinary Arts Akita University  Akita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"Gu","sequence":"additional","affiliation":[{"name":"Eye Institute and Department of Ophthalmology Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"NHC Key laboratory of Myopia and Related Eye Diseases, Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration Eye &amp; ENT Hospital, Fudan University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Cheng","sequence":"additional","affiliation":[{"name":"Eye Institute and Department of Ophthalmology Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"NHC Key laboratory of Myopia and Related Eye Diseases, Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration Eye &amp; ENT Hospital, Fudan University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjiang","family":"Xu","sequence":"additional","affiliation":[{"name":"Eye Institute and Department of Ophthalmology Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"NHC Key laboratory of Myopia and Related Eye Diseases, Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences Eye &amp; ENT Hospital, Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration Eye &amp; ENT Hospital, Fudan University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2003.06.020"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clae.2021.101559"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874364101711010252"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0000000000000408"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2020-316789"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2018.04.027"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0239939"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2015.08.017"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0000000000000662"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-8628(20000828)93:5<403::AID-AJMG11>3.0.CO;2-A"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-10867-4_79"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-1148"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2017.06.009"},{"issue":"1","key":"e_1_2_10_15_1","first-page":"109","article-title":"Linkage analysis of high\u2010density SNPs confirms keratoconus locus at 5q chromosomal region","volume":"37","author":"Bykhovskaya Y","year":"2016","journal-title":"Ophthalmic Genet"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/09273948.2020.1780271"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijo.IJO_3011_20"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2015.04.006"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/4740141"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-018-0071-8"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-021-00146-0"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1671-x"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.04.005"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0000000000002781"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.22641"},{"issue":"1","key":"e_1_2_10_26_1","first-page":"92","article-title":"Advances in research of genes involved in anthocyanin biological synthesis in plant and the genetic modification of the pathway","volume":"1","author":"De\u2010Yong Z","year":"2012","journal-title":"Journal of Tropical Organisms"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrestox.6b00423"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.03.012"},{"key":"e_1_2_10_29_1","first-page":"79","article-title":"Trafficking of immune cells in the cornea and ocular surface","volume":"5","author":"Qazi Y","year":"2012","journal-title":"Adv Ophthalmol"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9030531"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00427"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201646875"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00152-3"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/OPX.0000000000000455"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2023.109460"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-99805-9"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2021-318804"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3495"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/BS9.0000000000000026"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-016-0854-1"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1990"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1385\/IR:31:3:231"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C600321200"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-12-0194"},{"issue":"1","key":"e_1_2_10_45_1","first-page":"170","article-title":"Expression and significance of transcription factor EBF3 in tissues of human primary hepatocellular cancer","volume":"20","author":"Liping M","year":"2008","journal-title":"Chinese Journal of Clinical Laboratory Science"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2005.08.008"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2012.07.007"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.04.031"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-021-00678-9"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008130"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2021.110101"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2022.40.16_suppl.570"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gastha.2023.01.021"},{"key":"e_1_2_10_54_1","first-page":"178","article-title":"Hsa\u2010miR\u2010150\u20105p inhibits Wnt\u2010\u03b2\u2010catenin signaling in human corneal epithelial stem cells","volume":"28","author":"Kalaimani L","year":"2022","journal-title":"Mol Vis"},{"key":"e_1_2_10_55_1","volume-title":"A Histone Deacetylase Inhibitor:Entinostat","author":"Peng\u2010Kun LI","year":"2023"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-013-9550-y"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-001-2101-3"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.13.2497"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/dds197"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICB.0000000000001267"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12232681"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3121"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-024-2869-z"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70079","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T18:30:04Z","timestamp":1744396204000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70079"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":62,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1111\/jcmm.70079"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70079","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"2023-12-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70079"}}