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Emerging evidence suggests pyroptosis, a form of programmed cell death that is mediated by inflammasome, is implicated in persistent myofibroblast activation and fibrosis. Besides, numerous studies have demonstrated the effects of non\u2010coding RNAs on pyroptosis and myofibroblast activities. Herein, we aimed to target key long non\u2010coding RNA PVT1 with natural compound, carvacrol, to alleviate pyroptosis and myofibroblast activation in OSF. We first identified PVT1 was downregulated in the carvacrol\u2010treated fBMFs and then demonstrated that myofibroblast features and expression of pyroptosis makers were all reduced in response to carvacrol treatment. Subsequently, we analysed the expression of PVT1 and found that PVT1 was aberrantly upregulated in OSF specimens and positively correlated with several fibrosis markers. After revealing the suppressive effects of carvacrol on myofibroblast characterisitcs and pyroptosis were mediated by repression of PVT1, we then explored the potential mechanisms. Our data showed that PVT1 may serve as a sponge of microRNA(miR)\u201020a to mitigate the myofibroblast activation and pyroptosis. Altogether, these findings indicated that the anti\u2010fibrosis effects of carvacrol merit consideration and may be due to the attenuation of pyroptosis and myofibroblast activation by targeting the PVT1\/miR\u201020a axis.<\/jats:p>","DOI":"10.1111\/jcmm.70112","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T15:42:02Z","timestamp":1727278922000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Carvacrol inhibits the progression of oral submucous fibrosis via downregulation of <scp>PVT1<\/scp>\/<scp>miR<\/scp>\u201020a\u20105p\u2010mediated pyroptosis"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2720-3145","authenticated-orcid":false,"given":"Cheng\u2010Chia","family":"Yu","sequence":"first","affiliation":[{"name":"Institute of Oral Sciences, Chung Shan Medical University  Taichung Taiwan"},{"name":"Department of Dentistry Chung Shan 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