{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:31:02Z","timestamp":1788481862778,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T00:00:00Z","timestamp":1728000000000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pancreatic \u03b2\u2010cell damage mediated by apoptosis is believed to be a main trigger of type 1 diabetes mellitus (T1DM), which is proposed as an organ\u2010specific autoimmune disease mediated by T cells. Nonetheless, the fundamental origins of T1DM remain uncertain. Here, we illustrate that an increase in PLAGL1 expression induces \u03b2\u2010cell apoptosis, as evidenced by mitochondrial membrane impairment and nucleolar degradation. The gene expression levels from cDNA samples were determined using qRT\u2010PCR method. Western blot and Co\u2010immunoprecipitation were applied for protein expression and interactions, respectively. Flow cytometry and TUNEL assay were used to detect pancreatic \u03b2 cell apoptosis. Female NOD\/LtJ mice with recent\u2010onset T1DM has been used in <jats:italic>in vivo<\/jats:italic> studies. Glucose\u2010stimulated insulin secretion (GSIS) and glucose tolerance test (GTT) method is used for islet function assessment. Haematoxylin and Eosin (H&amp;E) and Immunohistochemistry (IHC) were performed to evalute histological improvement of islet beta. Subsequent cytoplasmic DNA accumulation triggers DNA senser, the cyclic guanosine monophosphate\u2010AMP synthase (cGAS)\u2010stimulator of interferon genes (STING) pathway. STING activation further stimulates downstream IRF3 and NF\u2010kB pathways, thus boost type\u2010I interferon signalling and NF\u2010kB mediated inflammation. These findings elucidate a molecular mechanism linking PLAGL1 induced cell apoptosis to type\u2010I interferon signalling and suggest a potential benefit for targeting cGAS\/STING in T1DM treatment.<\/jats:p>","DOI":"10.1111\/jcmm.70130","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T14:50:58Z","timestamp":1728053458000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>PLAGL1<\/scp> overexpression induces cytoplasmic <scp>DNA<\/scp> accumulation that triggers <scp>cGAS<\/scp>\/<scp>STING<\/scp> activation"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7349-6776","authenticated-orcid":false,"given":"Cheng","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pediatric Endocrinologic and Genetic and Metabolic Diseases Qingdao Women and Children's Hospital  Qingdao 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