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However, the regulatory mechanisms upstream of SIAT7A in this context remain poorly elucidated. Previous study demonstrated that KLF4 activates the <jats:italic>SIAT7A<\/jats:italic> gene in ischemic myocardium by binding to its promoter region. Nevertheless, the potential involvement of KLF4 in regulating SIAT7A expression in Ang II\u2010induced hypertrophic cardiomyocytes remains uncertain. This study seeks to deepen the underlying mechanisms of the KLF4 and SIAT7A interaction in the progression of Ang II\u2010induced cardiac hypertrophy. The results showed a concurrent increase in SIAT7A and KLF4 levels in hypertrophic myocardium of essential hypertension patients and in hypertrophic cardiomyocytes stimulated by Ang II. In\u00a0vitro experiments revealed that reducing KLF4 levels led to a decrease in both SIAT7A synthesis and Sialyl\u2010Tn antigen expression, consequently inhibiting Ang II\u2010induced cardiomyocyte hypertrophy. Intriguingly, reducing SIAT7A levels also resulted in decreased KLF4 expression and suppression cardiomyocyte hypertrophy. Consistent with this, elevating SIAT7A levels increased KLF4 expression and exacerbated cardiomyocyte hypertrophy in both in\u00a0vivo and in\u00a0vitro experiments. Additionally, a time\u2010course analysis indicated that KLF4 expression preceded that of SIAT7A. Luciferase reporter assays further confirmed that modulating <jats:italic>SIAT7A<\/jats:italic> levels directly influenced the transcriptional activity of <jats:italic>KLF4<\/jats:italic> in cardiomyocytes. In summary, KLF4 expression is upregulated in cardiomyocytes treated with Ang II, which subsequently induces the expression of SIAT7A. The elevated levels of SIAT7A, in turn, enhance the transcription of <jats:italic>KLF4<\/jats:italic>. These findings suggest a positive feedback loop between KLF4 and SIAT7A\u2010Sialyl\u2010Tn, ultimately promoting Ang II\u2010induced cardiac hypertrophy.<\/jats:p>","DOI":"10.1111\/jcmm.70144","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T11:40:36Z","timestamp":1729510836000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Role of <scp>KLF4<\/scp> and <scp>SIAT7A<\/scp> interaction accelerates myocardial hypertrophy induced by Ang <scp>II<\/scp>"],"prefix":"10.1111","volume":"28","author":[{"given":"Qiying","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Physiology Dalian Medical University  Dalian Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinrui","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Physiology Dalian Medical University  Dalian Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiantian","family":"Bian","sequence":"additional","affiliation":[{"name":"Department of Physiology Dalian Medical University  Dalian Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology Dalian Medical University  Dalian Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Cardiology The Second Affiliated Hospital of Dalian Medical University  Dalian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuesheng","family":"Lv","sequence":"additional","affiliation":[{"name":"Institute of Cancer Stem Cell Dalian Medical University  Dalian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shupeng","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Cardiology The Second Affiliated Hospital of Dalian Medical University  Dalian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Physiology Dalian Medical University  Dalian Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongmei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology Dalian Medical University  Dalian Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7147-8494","authenticated-orcid":false,"given":"Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiology The Second Affiliated Hospital of Dalian Medical University  Dalian China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(95)00606-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcad.2009.05.002"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-018-0007-y"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.828549"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.113.302501"},{"issue":"5","key":"e_1_2_9_7_1","first-page":"IV108","article-title":"Improvement of cardiac function by angiotensin converting enzyme inhibition. 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