{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T07:09:03Z","timestamp":1790492943786,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T00:00:00Z","timestamp":1730073600000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to investigate the role of 17\u03b2\u2010estradiol (E2)\u2010mediated oestrogen receptor (ER) in modulating the depressive\u2010like behaviours of ovariectomy (OVX) mice and the associated mechanisms. E2 was administrated in OVX mice. The behaviour and physiological changes of OVX mice including immobility time in tail suspension test (TST) and forced swimming test (FST), levels of serum E2, inflammatory mediators, oxidative stress factors, indoleamine2,3\u2010dioxygenase 1 (IDO1) and the neurotransmitters mediated by IDO1 activation were then recorded. Cell injury models established by lipopolysaccharide (LPS) or H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> stimulation in HT22 and BV2 cells were employed to further explore the mechanisms of E2's function. E2 treatment improved OVX\u2010induced increase of immobility time in FST and TST. Meanwhile, E2 ameliorated the changes of inflammatory factors (NF\u2010\u03baB, TNF\u2010\u03b1 and IL\u20106), IDO1, IDO1\u2010mediated TRP\/KYN pathway and oxidative stress factors (iNOS, MDA, GSH and SOD) in the hippocampus of OVX mice. Interestingly, ER\u03b2 inhibitor abolished E2's inhibitory effects on the inflammation and IDO1\u2010mediated TRP\/KYN pathway; ER\u03b2 inhibitor also abolished E2's anti\u2010oxidative stress effect. In cell experiments, ER\u03b2 small interfering RNA (siRNA) pretreatment reversed E2's anti\u2010inflammatory effect on LPS\u2010treated HT22 and BV2 cells and E2's inhibitory effect on IDO1 expression in LPS\u2010treated BV2 cells. ER\u03b2 siRNA pretreatment also reversed E2's anti\u2010oxidation effect on H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010treated HT22 cells. E2 exert the antidepressant function in OVX mice via ER\u03b2\u2010modulated suppression of NF\u2010\u03baB\u2010mediated inflammatory pathway, oxidative stress factors and IDO1\u2010mediated TRP\/KYN pathway in the hippocampus.<\/jats:p>","DOI":"10.1111\/jcmm.70179","type":"journal-article","created":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T03:23:56Z","timestamp":1730172236000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Effects of <scp>E2<\/scp> on the <scp>IDO1<\/scp>\u2010mediated metabolic <scp>KYN<\/scp> pathway in <scp>OVX<\/scp> female mice"],"prefix":"10.1111","volume":"28","author":[{"given":"Xi","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Pharmacy Zhejiang University Mingzhou Hospital  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefeng","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy 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