{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:37:36Z","timestamp":1753879056828,"version":"3.41.2"},"reference-count":38,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:00:00Z","timestamp":1731024000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acrylamide is broadly utilized in numerous areas with different purposes including being an additive, flocculating, sealing, dry strength improver and polymerizing agent, and so forth. Furthermore, it forms in certain food products at high temperatures. It poses serious hazard since its readily water\u2010soluble and very reactive nature. Besides in\u00a0vivo studies, several in\u00a0vitro studies with various cell lines are carried out to evaluate its toxicity. However, of these cell line studies, there are no mesothelium or mesothelioma cell lines. To fill this lacuna, we aimed at examining various dose range of acrylamide on SPC212 human mesothelioma cell line. First, we executed MTT and neutral red cytotoxicity tests and ascertained IC50 dose. Next, we performed inverted, light (haematoxylin\u2013eosin and May Gr\u00fcnwald), fluorescent (DAPI) and confocal microscope (AO\/EB) analyses as well as immunohistochemistry for Bax, Bcl\u20102 and PCNA proteins. As a result, we found IC50 of acrylamide at 2.65\u2009mM. Starting from 3.13\u2009mM of acrylamide dose, a deep decrease in cell proliferation was observed. Particularly in MTT assay, a proliferative action of acrylamide was detected at 0.39 and 0.78\u2009mM, supported with inverted microscope images. In light microscope analysis, several cellular degenerations, including condensed and kidney\u2010shaped nucleus were evident. In AO\/EB staining, cells with apoptotic characteristics augmented dose\u2010dependently, being upheld by a parallel uptick in Bax and a dimunition in Bcl\u20102 staining. Besides, PCNA decreased at IC50 dose of acrylamide. This is the acrylamide\u2010associated first study conducted on SPC212 mesothelioma cells encompassing advanced morphological analysis. We believe this study to be an incentive for future studies.<\/jats:p>","DOI":"10.1111\/jcmm.70190","type":"journal-article","created":{"date-parts":[[2024,11,9]],"date-time":"2024-11-09T03:39:14Z","timestamp":1731123554000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Morphological analysis and cytotoxicity of acrylamide on <scp>SPC212<\/scp> human mesothelioma cells: Do low doses induce proliferation, while high doses cause toxicity?"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0671-8529","authenticated-orcid":false,"given":"Sedat","family":"Kacar","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Eskisehir Osmangazi University  Eskisehir Turkey"},{"name":"Division of Pulmonary, Critical Care, Sleep and Occupational Medicine, Department of Medicine Indiana University  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ozlem","family":"Tomsuk","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Eskisehir Osmangazi University  Eskisehir Turkey"},{"name":"Department of Biotechnology and Biosafety, Graduate School of Natural and Applied Sciences Eskisehir Osmangazi University  Eskisehir Turkey"},{"name":"Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM) Eski\u015fehir Osmangazi University  Eski\u015fehir Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,8]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_9_2_1","DOI":"10.1021\/jf030204"},{"unstructured":"HamblinDO.The toxicity of acrylamide\u2014a preliminary report. Hommage Au Doyen Rene Fabre Membre De 1'Institut Professeur De Toxicologic A La Faculte De Pharmacie De Paris.1956: pp. 195\u20139.","key":"e_1_2_9_3_1"},{"unstructured":"IARC. International Agency for Reseach on Cancer.Monographs on the Evalution of the Carcinogenic Risk to Humans.1994;Vol: 60:pp. 389\u2013426.","key":"e_1_2_9_4_1"},{"doi-asserted-by":"publisher","key":"e_1_2_9_5_1","DOI":"10.5271\/sjweh.608"},{"doi-asserted-by":"publisher","key":"e_1_2_9_6_1","DOI":"10.1021\/jf020302f"},{"unstructured":"FAO\/WHO. Joint FAO\/WHO Consultation on Health Implications of Acrylamide in Food (2002: Geneva Switzerland) & WHO Food Safety Programme. (2002). Health implications of acrylamide in food: report of a joint FAO\/WHO consultation WHO Headquarters Geneva Switzerland 25\u201327 June 2002. 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