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Immune\u2010related differentially expressed genes in SIMI were identified through RNA sequencing and bioinformatics analysis. The expression levels of hub genes were detected using reverse transcription quantitative PCR. BMP10 was knocked down in the lipopolysaccharide\u2010induced mouse and cardiac microvascular endothelial cell (CMEC) models, and its functions were assessed by a series of in\u00a0vitro and in\u00a0vivo assays. Cell adhesion and HIF\u20101 pathway\u2010associated protein expressions were measured by western blot. Fenbendazole\u2010d3 was used to investigate whether BMP10 influenced SIMI development by modulating the HIF\u20101 pathway. Six key genes were screened, of which BMP10, HAMP, TRIM5, and MLANA were highly expressed, and PTPRN2 and AVP were lowly expressed. BMP10 knockdown ameliorated histopathological changes and inhibited apoptosis and CMEC immune infiltration in SIMI. BMP10 knockdown reduced inflammatory factor (IL\u20106, MCP\u20101, IFN\u2010\u03b2, and CCL11) levels and protein expressions of cell adhesion\u2010related molecules (VCAM\u20101 and ICAM\u20101). Mechanistically, the HIF\u20101 pathway agonist, Fenbendazole\u2010d3, significantly reversed the inhibitory effects of BMP10 knockdown on SIMI in\u00a0vitro, indicating that BMP10 knockdown impeded the development of SIMI by suppressing the HIF\u20101\u03b1 pathway. BMP10 knockdown blocks SIMI progression by inhibiting the HIF\u20101\u03b1 pathway, which provides a new potential therapeutic strategy for SIMI treatment.<\/jats:p>","DOI":"10.1111\/jcmm.70232","type":"journal-article","created":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T11:40:08Z","timestamp":1732880408000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["BMP10 Knockdown Modulates Endothelial Cell Immunoreactivity by Inhibiting the HIF\u20101\u03b1 Pathway in the Sepsis\u2010Induced Myocardial Injury"],"prefix":"10.1111","volume":"28","author":[{"given":"Huan","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Emergency Ganzhou People's Hospital  Ganzhou Jiangxi 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