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Calcium oxalate monohydrate (COM) crystals were prepared and used to treat HK\u20102 cells, which were further treated with different concentrations of linoleic acid in\u00a0vitro. Also, a small\u2010interfering RNA lentiviral vector of milk fat globule epidermal growth factor 8 (MFGE8) was constructed and transfected into HK\u20102 cells. The cell viability, level of intracellular ROS and autophagy were tested. In\u00a0vivo experiments were also carried out with a rat model for renal urolithiasis treated with linoleic acid. The results indicated that COM crystals promoted crystal deposition and apoptosis, increased levels of intracellular Ca<jats:sup>2+<\/jats:sup> and ROS levels and inhibited the proliferation of HK\u20102 cells. Linoleic acid exacerbated the damage of COM crystal\u2010treated HK\u20102 cells and renal tubular epithelial cells of the rat model for renal urolithiasis, which can be partially reversed by downregulation of MFGE8. These results collectively suggest that linoleic acid might enhance the damage of renal tubular epithelial cells caused by COM crystals.<\/jats:p>","DOI":"10.1111\/jcmm.70250","type":"journal-article","created":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T03:40:26Z","timestamp":1735270826000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Linoleic Acid Enhances Renal Tubular Epithelial Cells Autophagy Caused by Calcium Oxalate Monohydrate Crystals"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8448-9901","authenticated-orcid":false,"given":"Weiming","family":"Ma","sequence":"first","affiliation":[{"name":"Suzhou Medical College of Soochow University  Suzhou, Jiangsu China"},{"name":"Department of Urology Xuzhou Central Hospital  Xuzhou, Jiangsu 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