{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T02:27:38Z","timestamp":1775528858438,"version":"3.50.1"},"reference-count":81,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2022JJ30812"],"award-info":[{"award-number":["2022JJ30812"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Neuroinflammation, especially microglia\/macrophage activation, is a hallmark of spinal cord injury (SCI). Jisuikang (JSK) is a clinical experiential Chinese herbal formula for SCI therapy containing Huangqi (<jats:italic>Astragali Radix<\/jats:italic>), Danggui (<jats:italic>Angelica sinensis Radix<\/jats:italic>), Chishao (<jats:italic>Paeoniae Radix Rubra<\/jats:italic>), Dilong (earthworm, <jats:italic>Pheretima aspergillum<\/jats:italic>), Chuanxiong (<jats:italic>Chuanxiong Rhizoma<\/jats:italic>), Taoren (<jats:italic>Persicae Seman<\/jats:italic>) and Honghua (<jats:italic>Carthami Flos<\/jats:italic>). Eighteen active ingredients in 6 herbs of JSK were found to be correlated with inflammation, spinal injury and other diseases. These 18 active ingredients target 5464 genes according to the PubChem database. Through comparing differentially expressed genes between SCI and normal samples using GSE datasets, 50 hub genes were identified. These hub\u2010genes were enriched in oxidative stress response and inflammation response. The herb\u2010compound\u2010target, herb\u2010compound\u2010signalling and compound\u2010target\u2010signalling networks were generated and quercetin was identified as the hub compound. A concentration of 25\u2009\u03bcM quercetin showed no cytotoxicity but significantly protected microglial cells from LPS\u2010induced inhibition of cell viability. LPS stimulation elevated the levels of iNOS, IL\u20101\u03b2 and TNF\u2010\u03b1 but decreased IL\u201010 levels, whereas quercetin significantly attenuated LPS\u2010induced alterations in these factors. Moreover, quercetin targeted gene, IL1R1 was reduced by quercetin as predicted. Overexpression of IL1R1 further increased LPS\u2010induced inflammation, which could be partly reversed by quercetin treatment. In\u00a0vivo, quercetin improved histopathological alterations, inflammation and promoted M2 macrophage polarisation post\u2010injury, whereas IL1R1 overexpression partially attenuated the beneficial effects of quercetin on the rat SCI model. Collectively, quercetin, the main ingredient compound of JSK, protects against LPS\u2010induced cell viability inhibition and cellular inflammation, which could be partially attenuated by IL1R1 overexpression.<\/jats:p>","DOI":"10.1111\/jcmm.70269","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T13:55:21Z","timestamp":1734357321000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Pharmacological Network Analysis of the Functions and Mechanism of Quercetin From Jisuikang (<scp>JSK<\/scp>) in Spinal Cord Injury (<scp>SCI<\/scp>)"],"prefix":"10.1111","volume":"28","author":[{"given":"Lini","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Geriatrics, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoyu","family":"He","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zejun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunchao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guohua","family":"L\u00fc","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0301-6814","authenticated-orcid":false,"given":"Lei","family":"Kuang","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.112.659656"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2014.12.045"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2016.07.029"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2015.00054"},{"issue":"2","key":"e_1_2_9_6_1","first-page":"73","article-title":"Microglia and Ischemic Stroke: A Double\u2010Edged Sword","volume":"5","author":"Patel A. R.","year":"2013","journal-title":"International Journal of Physiology, Pathophysiology and Pharmacology"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2020.113310"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.07.027"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1146-14.2014"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110136"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/8958721"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0254-6272(16)30081-4"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.215264"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/9542359"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.162767"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1179\/2045772314Y.0000000273"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2018.09.028"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.5414\/CP202613"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.297087"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12951"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.118"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-023-04137-z"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21214"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/1646905"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiolchem.2020.107402"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000031023"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.113097"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758-2946-6-13"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27175481"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms13066964"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2019.10.014"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09234-6"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq675"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.1c00203"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.42539"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/sc.2009.111"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.1995.12.1"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.963582"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031731"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-014-9070-5"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri978"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2015.4268"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-008-9006-x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.533841"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2021.173878"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7028"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2021.03.037"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-019-1613-2"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/sc.2010.45"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1089\/089771503767168500"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcma.2018.03.014"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.01076"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.27136"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.324857"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2018.05.022"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2017.4021"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2016.00047"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.13275"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13070581"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2010.11.014"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2006.11.074"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2008.03.009"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1385\/JMN:29:1:45"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2007.12.037"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2010.10.044"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5087-12.2013"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2691"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3800"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12606"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2009.11.007"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.24.11393"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2008.09.002"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0730-12.2012"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0176-07.2007"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1177\/09727531211046367"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-012-0801-6"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature21029"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2011.05.011"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33463-x"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2024.05.028"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12010149"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.01.008"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70269","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T05:09:02Z","timestamp":1735621742000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":81,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1111\/jcmm.70269"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70269","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"2023-11-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70269"}}