{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:09:28Z","timestamp":1787015368278,"version":"build-2736575974"},"reference-count":59,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T00:00:00Z","timestamp":1733788800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82374488"],"award-info":[{"award-number":["82374488"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82174403"],"award-info":[{"award-number":["82174403"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82374467"],"award-info":[{"award-number":["82374467"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    HBP\u2010A is the main active component of a traditional Chinese medicine\n                    <jats:italic>Huaizhen Yanggan Capsule,<\/jats:italic>\n                    for the remarkable treatment of knee osteoarthritis (KOA). This study aimed to elucidate the ameliorative effect of HBP\u2010A on meniscus hypertrophy and mineralisation in KOA and the molecular mechanism of its action. An Hartley guinea pig model of KOA that underwent anterior cruciate ligament transection (ACLT) and a model of rat primary meniscus fibrochondrocytes (PMFs) were used to investigate the ameliorative effect of HBP\u2010A on meniscal hypertrophy and calcification and its signal transduction mechanism of action. The results show that Guinea pig's meniscus width, as well as the area of meniscus calcification and meniscus and articular cartilage injury score, were significantly reduced in the HBP\u2010A intervention group compared to the ACLT group. The expression levels of mtrix metalloproteinase 13 (MMP13), runt\u2010related transcription factor 2 (Runx2), Indian hedgehog (Ihh), alkaline phosphatase (ALP), and ankylosis homologue (ANKH) at the protein and gene level significantly decreased in the HBP\u2010A intervention group compared to the ACLT group. In\u00a0vitro study, apoptosis, hypertrophy, and calcification of rat PMFs after 10% stretch force were significantly improved with HBP\u2010A intervention. Western blot and RT\u2010qPCR showed that hypertrophy, calcification, and p38 MAPK signalling pathway\u2010related markers of PMFs were incredibly depressed in the HBP\u2010A intervention group compared to the 10% stretch force group. In conclusion, HBP\u2010A can slow down meniscus hypertrophy and mineralisation induced by abnormal mechanical loading, and its mechanism of action may be through the p38\u2010MAPK signalling pathway.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70271","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T12:39:16Z","timestamp":1733834356000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Molecular Mechanism Investigation of\n                    <scp>HBP<\/scp>\n                    \u2010A Slows Down Meniscus Hypertrophy and Mineralisation by the Damage Mechanical Model"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5643-8311","authenticated-orcid":false,"given":"Zongrui","family":"Yang","sequence":"first","affiliation":[{"name":"Shi's Center of Orthopedics and Traumatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Institute of Traumatology &amp; Orthopedics Shanghai Academy of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingcai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shi's Center of Orthopedics and Traumatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Institute of Traumatology &amp; Orthopedics Shanghai Academy of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongming","family":"Liu","sequence":"additional","affiliation":[{"name":"Shi's Center of Orthopedics and Traumatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Institute of Traumatology &amp; Orthopedics Shanghai Academy of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhe","family":"Xiong","sequence":"additional","affiliation":[{"name":"Shi's Center of Orthopedics and Traumatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Institute of Traumatology &amp; Orthopedics Shanghai Academy of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Shi's Center of Orthopedics and Traumatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Institute of Traumatology &amp; Orthopedics Shanghai Academy of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Shi","sequence":"additional","affiliation":[{"name":"Shi's Center of Orthopedics and Traumatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Institute of Traumatology &amp; Orthopedics Shanghai Academy of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Chen","sequence":"additional","affiliation":[{"name":"Shi's Center of Orthopedics and Traumatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Institute of Traumatology &amp; Orthopedics Shanghai Academy of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengming","family":"Wang","sequence":"additional","affiliation":[{"name":"Shi's Center of Orthopedics and Traumatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Institute of Traumatology &amp; Orthopedics Shanghai Academy of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiya","family":"Ge","sequence":"additional","affiliation":[{"name":"Shi's Center of Orthopedics and Traumatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Institute of Traumatology &amp; Orthopedics Shanghai Academy of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongsheng","family":"Zhan","sequence":"additional","affiliation":[{"name":"Shi's Center of Orthopedics and Traumatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Institute of Traumatology &amp; Orthopedics Shanghai Academy of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhibi","family":"Shen","sequence":"additional","affiliation":[{"name":"Shi's Center of Orthopedics and Traumatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Institute of Traumatology &amp; Orthopedics Shanghai Academy of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4089-7599","authenticated-orcid":false,"given":"Guoqing","family":"Du","sequence":"additional","affiliation":[{"name":"Shi's Center of Orthopedics and Traumatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Institute of Traumatology &amp; Orthopedics Shanghai Academy of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2016.11.013"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.42089"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00264-016-3370-5"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.835730"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052619"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2022.10.003"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115552"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering11020110"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2022.1001212"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-020-03367-2"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10061462"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0167733"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008207.2019.1702031"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2015.02.008"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-060418-052547"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-014-0448-1"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-022-01093-3"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2012.10.003"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874325001610010690"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjsports-2022-105496"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.754421"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546515621285"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-012-0500-4"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11162600"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e17-02-0087"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2014.11.003"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1302\/2046-3758.92.BJR-2019-0172.R1"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0617-7"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-013-1511-x"},{"key":"e_1_2_10_31_1","unstructured":"AVMA Guidelines for the Euthanasia of Animals. 2024: The AVMA Guidelines for the Euthanasia of Animals Provide Veterinarians Guidance in Relieving Pain and Suffering of Animals That Are to Be Euthanized."},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2474-11-19"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2010.04.015"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.934088"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/189516"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.53009"},{"issue":"15","key":"e_1_2_10_37_1","first-page":"2829","article-title":"Culture and Characterization of Rat Meniscus Fibrochondrocytes In\u00a0Vitro","volume":"19","author":"Guo\u2010Qing D. U.","year":"2019","journal-title":"Progress in Modern Biomedicine"},{"issue":"6","key":"e_1_2_10_38_1","first-page":"461","article-title":"Regulating Effect of Anodonta Glucan HBP\u2010A on Chondrocytes Through Wnt Pathway","volume":"27","author":"Wei S. P.","year":"2014","journal-title":"Zhongguo Gu Shang"},{"key":"e_1_2_10_39_1","volume-title":"Study on the Mechanism of Liver\u2010Softening Herbs\u2010Huaizhen Yanggan Capsule\u2010For Treating Osteoarthritis","author":"Ningyang G.","year":"2011"},{"key":"e_1_2_10_40_1","unstructured":"C.Yuelong W.Xiang P.Jian et\u00a0al. \u201cClinical Study on the Treatment of Osteoarthritis of the Knee by Huaizhen Yanggan Capsule \u201d In: The Third Annual Meeting of the Fourth Session of Traumatology & Orthopedics Branch of the China Association of Chinese Medicine and the Experience Exchange Meeting of the Traumatology & Orthopedics Collaboration Group for the Construction of Key Specialties (Diseases) of the Eleventh Five\u2010Year Plan of the State Administration of Traditional Chinese Medicine. Fuyang China(2008)."},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.12111439"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-020-02261-5"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2016.08.004"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2022.08.002"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00256-022-04191-6"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00232.2014"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-015-2277-6"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21228660"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.540591"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.07.034"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21061913"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21124258"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11101444"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2009.06.005"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-00322-w"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2474-15-438"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.560117"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S365545"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-014-0491-3"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-024-01190-6"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T05:19:28Z","timestamp":1735190368000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":59,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1111\/jcmm.70271"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70271","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"2024-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70271"}}