{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T06:25:56Z","timestamp":1770272756740,"version":"3.49.0"},"reference-count":62,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T00:00:00Z","timestamp":1734048000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The disruption of nerve parenchyma and axonal networks triggered by spinal cord injury (SCI) can initiate a cascade of events associated with secondary injury. Toll\u2010like receptors play a critical role in initiating and regulating immune\u2010inflammatory responses following SCI; however, the precise involvement of Toll\u2010like receptor\u20103 (TLR3) in secondary neuronal injury remains incompletely understood. To investigate the potential contribution of TLR3 in mediating neuronal pressure\u2010induced damage, we established a stress\u2010induced neuronal damage model using rat anterior horn motor neuron line (VSC4.1), which was subjected to varying levels and durations of sustained pressure. Our findings suggest that pressure induces neuronal damage and apoptosis, and reduced proliferation rates in VSC4.1 cells. Furthermore, this pressure\u2010induced neuronal injury is accompanied by upregulation of TLR3 expression and activation of downstream TLR3 signalling molecules. Knockdown experiments targeting TLR3 significantly alleviate pressure\u2010induced motor neuron injury and apoptosis within the anterior horn region while promoting mitochondria\u2010related autophagy and reducing mitochondrial dysfunction via the TLR3\/IRF3 and TLR3\/NF\u2010\u03baB pathways.<\/jats:p>","DOI":"10.1111\/jcmm.70276","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T17:20:22Z","timestamp":1734110422000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["TLR3 Knockdown Attenuates Pressure\u2010Induced Neuronal Damage In\u00a0Vitro"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0234-3404","authenticated-orcid":false,"given":"Li","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Neurology Xuzhou Medical University  Xuzhou China"},{"name":"Department of Neurology Benq Hospital Affiliated to Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongzhong","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Benq Hospital Affiliated to Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Neurology Xuzhou Medical University  Xuzhou China"},{"name":"Department of Neurology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taiyang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Neurology Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuting","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Neurology Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Neurology Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Neurology Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongtao","family":"Lin","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoqing","family":"Gu","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Neurology Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Neurology Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoliang","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Neurology Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Hua","sequence":"additional","affiliation":[{"name":"Department of Neurology Xuzhou Medical University  Xuzhou China"},{"name":"Department of Interdisciplinary Health Sciences College of Allied Health Sciences, Augusta University  Augusta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12859"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.350210"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3344\/kjp.2022.35.3.291"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004010051187"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-11-40"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.sc.3101674"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1227\/00006123-199302000-00016"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119789"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2005.22.398"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.13721"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13356-2"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07603-1"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207533"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2014.2927"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10113019"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.25120"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2024.1422357"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3344\/kjp.2019.32.1.12"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12588"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.01.022"},{"issue":"4","key":"e_1_2_9_22_1","first-page":"3703","article-title":"miRNA\u2010146a Attenuates Inflammation in an In\u00a0Vitro Spinal Cord Injury Model via Inhibition of TLR4 Signaling","volume":"16","author":"Tan Y.","year":"2018","journal-title":"Experimental and Therapeutic Medicine"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2022.974890"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2022.2111083"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9020381"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2019.10.013"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2019.06.031"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-016-0031-z"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamasurg.2024.1043"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.112188"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7765"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/joim.12957"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2023.01.039"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.wneu.2024.01.084"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2023.114475"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmpb.2021.106476"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2024.04.004"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2019.05.004"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1011647"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1139522"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1515\/revneuro-2014-0052"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.B2000808"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhac461"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2021.04.016"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1385\/JMN:29:3:185"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490330402"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201902122RR"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2023.02.021"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aao6047"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09397-2"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11111855"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-023-01151-1"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12010166"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1822097"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100818-125242"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1201940"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.79876"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-31213-7"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.4600"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00058.2021"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35501-0"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-05053-8"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.322933"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70276","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T10:18:02Z","timestamp":1735208282000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70276"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":62,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1111\/jcmm.70276"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70276","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"2024-05-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70276"}}