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We explored alterations in pulmonary artery endothelial cells under the hypoxic and elevated interleukin\u201017 conditions that are commonly present in patients with chronic thromboembolic pulmonary hypertension. We measured the serum interleukin\u201017 levels in 10 chronic thromboembolic pulmonary hypertension patients and 10 healthy control persons. The expressions and localisations of hypoxia\u2010inducible factor\u20101\u03b1 and endothelial nitric oxide synthase were detected in tissues. The levels of hypoxia\u2010inducible factor\u20101\u03b1, endothelial nitric oxide synthase, nitric oxide, and reactive oxygen species in cultured pulmonary artery endothelial cells were examined under hypoxia and\/or interleukin\u201017 treatment. The serum interleukin\u201017 level was increased in chronic thromboembolic pulmonary hypertension patients. Hypoxia\u2010inducible factor\u20101\u03b1 was increased, and endothelial nitric oxide synthase was decreased in chronic thromboembolic pulmonary hypertension pulmonary vascular tissue. After receiving the hypoxia combined with interleukin\u201017 treatment, pulmonary artery endothelial cells showed increased levels of hypoxia\u2010inducible factor\u20101\u03b1 and phospho\u2010endothelial nitric oxide synthase (Thr495) (<jats:italic>p<\/jats:italic>\u2009=\u20090.001 and 0.063, respectively) and a decreased level of endothelial nitric oxide synthase (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). In addition, the nitric oxide level was significantly decreased (<jats:italic>p<\/jats:italic>\u2009=\u20090.001), whereas the reactive oxygen species level was insignificantly increased in pulmonary artery endothelial cells. Chronic thromboembolic pulmonary hypertension patients might experience increased inflammation and hypoxia due to dysregulation of the hypoxia\u2010inducible factor\u20101\u03b1\/endothelial nitric synthase pathway in pulmonary artery endothelial cells under inflammation and hypoxia, contributing to the pathogenesis of chronic thromboembolic pulmonary hypertension.<\/jats:p>","DOI":"10.1111\/jcmm.70289","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T17:45:10Z","timestamp":1737135910000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Hypoxia Combined With Interleukin\u201017 Regulates Hypoxia\u2010Inducible Factor\u20101\u03b1\/Endothelial Nitric Oxide Synthase Expression in Pulmonary Artery Endothelial Cells"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5832-7458","authenticated-orcid":false,"given":"Shuai","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"},{"name":"Medical Research Center, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianjun","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"},{"name":"Medical Research Center, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juanni","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suqiao","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jifeng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuguang","family":"Kuang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"},{"name":"Medical Research Center, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanhua","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing 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