{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T07:04:14Z","timestamp":1793343854745,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2024,12,25]],"date-time":"2024-12-25T00:00:00Z","timestamp":1735084800000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018609","name":"High-level Hospital Construction Project of Guangdong Provincial People's Hospital","doi-asserted-by":"publisher","award":["DFJHBF202104"],"award-info":[{"award-number":["DFJHBF202104"]}],"id":[{"id":"10.13039\/501100018609","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Aortic dissection (AD) represents a critical condition characterised by a tear in the inner lining of the aorta, leading to the leakage of blood into the layers of the aortic wall, posing a significant risk to life. However, the pathogenesis is unclear. In this study, scRNA\u2010seq was applied to cells derived from aortas of both AD and non\u2010AD donors (control) to unveil the cellular landscape. ScRNA\u2010seq data uncover significant cellular heterogeneity in AD aortas. Specifically, we observed an accumulation of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells, which contributed to inflammation and cell death, and abnormal collagen formation mediated by fibroblast cells in AD. Moreover, we revealed a greater prevalence of cell death, oxidative stress and senescence in AD aorta cells. Furthermore, we found a decrease in the percentage of vascular stem cells (VSCs), along with a repression in their ability to differentiate into contractile vascular smooth muscle cells (VSMCs). Finally, our data demonstrated that the PDGF signalling pathway was activated in AD. We found that PDGF activation could lead to VSMCs aberrant switch from contractile to synthetic phenotype, which could be ameliorated by PDGF inhibitor. This underscores the potential of the PDGF as a therapeutic target for AD. In summary, our study highlights the cellular heterogeneity and associated injuries within aortas affected by AD, including cell death, oxidative stress, senescence and dysregulation of signalling pathways influencing the aberrant phenotypic switch of VSMCs. These insights offer valuable contributions to understanding the molecular mechanisms underlying AD and present new avenues for therapeutic intervention in this condition.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70293","type":"journal-article","created":{"date-parts":[[2024,12,25]],"date-time":"2024-12-25T07:35:27Z","timestamp":1735112127000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Single\u2010Cell\n                    <scp>RNA<\/scp>\n                    \u2010Seq Reveals Injuries in Aortic Dissection and Identifies\n                    <scp>PDGF<\/scp>\n                    Signalling Pathway as a Potential Therapeutic Target"],"prefix":"10.1111","volume":"28","author":[{"given":"Yichi","family":"Han","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences) Southern Medical University  Guangzhou Guangdong China"},{"name":"Department of Critical Care Medicine Chengdu Fifth People's Hospital (The Second Clinical Medical College, Afliated Fifth People's Hospital of Chengdu University of Traditional Chinese Medicine)  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongji","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences) Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juli","family":"Liu","sequence":"additional","affiliation":[{"name":"Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences) Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingchen","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine South 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