{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:26:50Z","timestamp":1785410810268,"version":"3.56.0"},"reference-count":32,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2024,12,25]],"date-time":"2024-12-25T00:00:00Z","timestamp":1735084800000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100020950","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 112\u20102314\u2010B\u2010005 \u2010011"],"award-info":[{"award-number":["NSTC 112\u20102314\u2010B\u2010005 \u2010011"]}],"id":[{"id":"10.13039\/501100020950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010101","name":"Taichung Veterans General Hospital","doi-asserted-by":"publisher","award":["TCVGH\u2010NHRI111009"],"award-info":[{"award-number":["TCVGH\u2010NHRI111009"]}],"id":[{"id":"10.13039\/501100010101","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010101","name":"Taichung Veterans General Hospital","doi-asserted-by":"publisher","award":["TCVGH\u2010YM1120101"],"award-info":[{"award-number":["TCVGH\u2010YM1120101"]}],"id":[{"id":"10.13039\/501100010101","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013227","name":"Health Promotion Administration, Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["MOHW112\u2010TDU\u2010B\u2010222\u2010124014"],"award-info":[{"award-number":["MOHW112\u2010TDU\u2010B\u2010222\u2010124014"]}],"id":[{"id":"10.13039\/100013227","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Leukaemia stem cells (LSCs) are major contributors to chemoresistance in acute myeloid leukaemia (AML). Identifying potential biomarkers within LSCs that can predict chemosensitivity in AML is key. This prospective study involved 20 consecutive de novo AML patients who underwent \u20187\u2009+\u20093\u2019 induction therapy. The patients were divided into CR (<jats:italic>n<\/jats:italic>\u2009=\u200915) and non\u2010CR (<jats:italic>n<\/jats:italic>\u2009=\u20095) groups. Using single\u2010cell RNA sequencing, we examined the cellular states of bone marrow mononuclear cells from AML patients at diagnosis and identified LSC among these cells. Our results showed that in non\u2010CR AML patients, a significant increase in the proportion of immature cells during haematopoiesis within the AML cell populations was observed. Moreover, the expression of myeloperoxidase (MPO) (log<jats:sub>2<\/jats:sub> fold\u2010change\u2009=\u20090.89; adjusted <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) and thyrotropin\u2010releasing hormone (TRH) (log<jats:sub>2<\/jats:sub> fold\u2010change\u2009=\u20090.65; adjusted <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) was higher within LSCs in the CR group than in the non\u2010CR group. Furthermore, patients with higher expression of MPO and TRH demonstrated improved relapse\u2010free survival (<jats:italic>p<\/jats:italic>\u2009=\u20090.002 for MPO; <jats:italic>p<\/jats:italic>\u2009=\u20090.009 for TRH) and overall survival (<jats:italic>p<\/jats:italic>\u2009=\u20090.002 for MPO; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001 for TRH). The connection between MPO or TRH and chemosensitivity could be linked with the downregulation of transforming growth factor and the upregulation of interferon\u2010\u03b1. In conclusion, MPO and TRH in LSCs could serve as chemosensitivity biomarkers in AML.<\/jats:p>","DOI":"10.1111\/jcmm.70306","type":"journal-article","created":{"date-parts":[[2024,12,25]],"date-time":"2024-12-25T12:33:38Z","timestamp":1735130018000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Myeloperoxidase and Thyrotropin\u2010Releasing Hormone Within Leukaemia Stem Cells Increased Chemosensitivity in Acute Myeloid Leukaemia"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1205-7368","authenticated-orcid":false,"given":"Chung\u2010Hsing","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Mathematics University of Taipei  Taipei Taiwan"},{"name":"National Institute of Cancer Research National Health Research Institutes  Zhunan 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Veterans General Hospital  Taichung Taiwan"},{"name":"Department of Public Health Fu Jen Catholic University  New Taipei City Taiwan"},{"name":"Institute of Genomics and Bioinformatics National Chung Hsing University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u2010Mei Maureen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry National Chi Nan University  Puli Nantou Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi\u2010Ying F.","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Biopharmaceutical Sciences National Yang\u2010Ming Chiao Tung University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po\u2010Liang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Medical Research Taichung Veterans General Hospital  Taichung Taiwan"},{"name":"Department of Pathology and Laboratory Medicine, Perelman School of Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Rung","family":"Tsai","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Medical Oncology, Department of Medicine Taichung Veterans General Hospital  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9744-0368","authenticated-orcid":false,"given":"Chieh\u2010Lin Jerry","family":"Teng","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Medical Oncology, Department of Medicine Taichung Veterans General Hospital  Taichung Taiwan"},{"name":"Department of Post\u2010Baccalaureate Medicine, College of Medicine National Chung Hsing University  Taichung Taiwan"},{"name":"School of Medicine Chung Shan Medical University  Taichung Taiwan"},{"name":"Department of Life Science Tunghai University  Taichung Taiwan"},{"name":"Program in 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