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Recently, targeting the PD\u20101 pathway with immunotherapies has shown promise as a novel glioma treatment. However, not all patients experience long\u2010lasting benefits, underscoring the necessity to discover reliable biomarkers for predicting treatment outcomes. This study applied a range of advanced artificial intelligence methods to identify a new biomarker linked to the effectiveness of anti\u2010PD\u20101 immunotherapy in glioma patients. Through an extensive analysis of single\u2010cell RNA sequencing and bulk transcriptomic data from over 3000 patients, the gene SLFN12 emerged as a significant and independent predictor of immunotherapy response. Our results indicate that elevated SLFN12 expression is associated with worse overall survival across various glioma cohorts. Notably, we found that patients with high SLFN12 levels are less likely to respond favourably to anti\u2010PD\u20101 treatment, positioning SLFN12 as a clinically valuable biomarker for personalised treatment decisions. Functional studies revealed that SLFN12 is involved in key immune\u2010related pathways, shedding light on its potential role in altering the tumour microenvironment and impacting immunotherapy outcomes. Additional laboratory experiments confirmed the role of SLFN12 in promoting glioma cell proliferation, migration and macrophage recruitment. In summary, this study identifies SLFN12 as a novel biomarker for predicting immunotherapy response in glioma patients, offering new insights for precision immunotherapy approaches.<\/jats:p>","DOI":"10.1111\/jcmm.70317","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T04:49:51Z","timestamp":1735620591000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deciphering the Role of <scp>SLFN12<\/scp>: A Novel Biomarker for Predicting Immunotherapy Outcomes in Glioma Patients Through Artificial Intelligence"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6538-7830","authenticated-orcid":false,"given":"Zigui","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Haikou Affiliated Hospital of Central South University Xiangya School of Medicine  Haikou 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