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In this study, we exploited an ultrabright semiconducting polymer dots (Pdots) integrating with a three\u2010dimensional (3D) near\u2010infrared\u2010II (NIR\u2010II) fluorescence imaging system designed to assess the efficacy of potent anti\u2010angiogenic agents PX\u2010478 and BPR0C261 in an oral squamous cell carcinoma (OSCC) tumour model, which depends on angiogenesis for dissemination. PX\u2010478, a hypoxia\u2010inducible factor\u20101\u03b1 (HIF\u20101\u03b1) inhibitor, and BPR0C261, a microtubule\u2010disrupting agent, were administrated into tumour\u2010bearing mice established using murine MTCQ1 tongue cancer cells through intraperitoneal injection and oral gavage, respectively. Our findings showed that PX\u2010478 and BPR0C261 significantly inhibited tumour growth and extended the life span of tumour\u2010bearing mice without decreasing the body weights. The Pdots\u2010based NIR\u2010II vascular imaging demonstrated that the tumour vascularity was suppressed by PX\u2010478 and BPRC0261. Accordingly, the excised tumours treated with anti\u2010angiogenic agents showed less blood vessels than that treated with vehicles. The expression of endothelial markers CD31 was also found to be reduced in tumours treated with PX\u2010478 and BPRC0261 using immunohistochemical (IHC) staining and Western blot analysis. Furthermore, PX\u2010478 could suppress the expression of HIF\u20101\u03b1 and vascular endothelial growth factor\u2010A (VEGF\u2010A), but BPRC0261 only suppressed VEGF\u2010A. Taken together, this innovative 3D NIR\u2010II imaging system combining the biocompatible Pdots with unique optical specificity enables non\u2010invasive, real\u2010time monitoring the efficacy of anti\u2010angiogenic compounds.<\/jats:p>","DOI":"10.1111\/jcmm.70324","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T04:52:01Z","timestamp":1736139121000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Integrating Ultrabright Polymer Dots and Stereo NIR\u2010II Imager for Assessing Anti\u2010Angiogenic Drugs in Oral Cancer Model"],"prefix":"10.1111","volume":"29","author":[{"given":"Bo\u2010Han","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Biomedical Imaging and Radiological Sciences National Yang Ming Chiao Tung University  Taipei 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