{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T17:35:47Z","timestamp":1789148147088,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T00:00:00Z","timestamp":1738108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003785","name":"Guangdong Medical Research Foundation","doi-asserted-by":"publisher","award":["A2024307"],"award-info":[{"award-number":["A2024307"]}],"id":[{"id":"10.13039\/501100003785","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022A1515010776"],"award-info":[{"award-number":["2022A1515010776"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Reduced trophoblast migration and invasion contribute to unexplained recurrent spontaneous abortion (URSA). Aquaporin 3 (AQP3) plays a crucial role in facilitating trophoblast migration and invasion during early pregnancy through fetal\u2010maternal crosstalk. This study aimed to comprehensively investigate the mechanism involving AQP3 and its modulatory effects on human extravillous trophoblast (HTR\u20108\/SVneo cells) migration and invasion. AQP3 and IGF2BP1 expression was analysed using immunohistochemistry and quantitative real\u2010time polymerase chain reaction. The AQP3\u2010associated molecular mechanisms were explored using western blot, meRIP, RNA stability assays and RNA\u2010protein pull\u2010down experiments. Furthermore, the role of IGF2BP1 in HTR\u20108\/SVneo cells was assessed using transwell assays. AQP3 and IGF2BP1 expression was lower in the chorionic villi samples of the URSA group than in those of the control group. AQP3 was involved in regulating the activation of the PI3K\/AKT signalling pathway. Additionally, METTL14 interacted with AQP3 mRNA, thereby influencing its stability. Furthermore, AQP3 mRNA bound to the IGF2BP1 protein, and IGF2BP1 knockdown resulted in reduced AQP3 mRNA stability and impaired trophoblast migration and invasion. METTL14 and IGF2BP1 stabilise AQP3 mRNA expression by mediating m6A, thereby facilitating HTR\u20108\/SVneo cell migration and invasion via the PI3K\/AKT signalling pathway. Targeting AQP3 could potentially contribute to strategies aimed at mitigating URSA development.<\/jats:p>","DOI":"10.1111\/jcmm.70325","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T07:50:17Z","timestamp":1738137017000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<scp>AQP3<\/scp>\n                    Influences Unexplained Recurrent Abortion by Regulating Trophoblast Cell Migration and Invasion via the\n                    <scp>METTL14<\/scp>\n                    \/\n                    <scp>IGF2BP1<\/scp>\n                    \/\n                    <scp>AQP3<\/scp>\n                    \/\n                    <scp>PI3K<\/scp>\n                    \/\n                    <scp>AKT<\/scp>\n                    Pathway"],"prefix":"10.1111","volume":"29","author":[{"given":"Yingqi","family":"Nong","sequence":"first","affiliation":[{"name":"Department of Reproductive Health and Infertility Guangdong Women and Children Hospital  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyi","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Traumatic Orthopedics, ZhuJiang Hospital Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Reproductive Health and Infertility Guangdong Women and Children Hospital  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Reproductive Health and Infertility Guangdong Women and Children Hospital  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Graduate School of Guangzhou Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyin","family":"Lv","sequence":"additional","affiliation":[{"name":"Graduate School of Guangzhou Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zitao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Reproductive Health and Infertility Guangdong Women and Children Hospital  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiqian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Reproductive Health and Infertility Guangdong Women and Children Hospital  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3860-9466","authenticated-orcid":false,"given":"Fenghua","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Reproductive Health and Infertility Guangdong Women and Children Hospital  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,29]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"653","article-title":"Expert Consensus on the Diagnosis and Treatment of Recurrent Miscarriage","volume":"57","author":"Obstetrics Group, Chinese Medical Association Obstetrics and Gynecology Branch","year":"2022","journal-title":"Zhonghua Fu Chan Ke Za Zhi"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-020-00228-z"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28593"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2018.12.011"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8111316"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-013-9965-5"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/plac.2001.0717"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2017.09.010"},{"issue":"4","key":"e_1_2_11_10_1","first-page":"1014","article-title":"Aquaporin Expression in Breast Cancer and Their Involvement in Bleb Formation, Cell Motility and Invasion in Endocrine Resistant Variant Cells","volume":"56","author":"Ahmad A. E.","year":"2020","journal-title":"International Journal of Oncology"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.03.133"},{"issue":"1","key":"e_1_2_11_12_1","first-page":"713","article-title":"Downregulation of Aquaporin 3 Inhibits Cellular Proliferation, Migration and Invasion in the MDA\u2010MB\u2010231 Breast Cancer Cell Line","volume":"16","author":"Arif M.","year":"2018","journal-title":"Oncology Letters"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dey290"},{"issue":"4","key":"e_1_2_11_14_1","first-page":"1493","article-title":"MicroRNA\u2010488 Inhibits Proliferation, Invasion and EMT in Osteosarcoma Cell Lines by Targeting Aquaporin 3","volume":"53","author":"Qiu J.","year":"2018","journal-title":"International Journal of Oncology"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719116657893"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-021-00726-z"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2020.02.004"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3724"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.31868"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0168-5"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-021-01359-x"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15403-9"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.earlhumdev.2012.01.009"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2021.103321"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28112"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11072132"},{"issue":"1","key":"e_1_2_11_27_1","first-page":"79","article-title":"miR\u201034a\u20105p Regulates Viability, Invasion and Apoptosis of Placental Trophoblastic Cells via Modulating CDK6 and PI3K\/AKT Pathway","volume":"40","author":"Li Q.","year":"2020","journal-title":"Nan Fang Yi Ke Da Xue Xue Bao"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70325","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T09:05:24Z","timestamp":1750151124000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70325"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,29]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1111\/jcmm.70325"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70325","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,29]]},"assertion":[{"value":"2024-07-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70325"}}