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For this purpose, oleuropein, hesperidin, piperine, proanthocyanidins and retinoic acid, which have previously been proven to be bioactive components, anti\u2010angiogenic performances were experimentally tested in retinal pigment epithelial cells. Bioactive ingredients were applied to retinal pigment epithelial cells at varying doses for 48\u2009h, and then IC<jats:sub>50<\/jats:sub> doses were calculated by MTT analysis. VEGFA and VEGFR1 protein levels were measured by ELISA analysis. Using the RT\u2010PCR technique, the mRNA expression levels of EGF, EGFR, PDGF, PDGFR\u2010\u03b2 and HIF1A were analysed. Among all bioactive compounds, the bevacizumab group showed the best anti\u2010VEGF activity, followed by the proanthocyanidins and piperine groups. Piperine group showed EGF, PDGF and HIF1A expressions; proanthocyanidins, on the other hand, reduced EGFR, PDGF and HIF1A expressions. Retinoic acid showed an angiogenic effect by increasing VEGFA protein levels and EGF and PDGFR\u2010\u03b2 expressions. Although hesperidin increased VEGFA protein levels, it decreased EGF, PDGF, PFGFR\u2010\u03b2 and HIF1A expression levels. Among the bioactive components stated in previous studies to have anti\u2010angiogenic properties, only proanthocyanidins and piperin had anti\u2010VEGF properties. Considering that angiogenesis does not proceed only through VEGF, this study investigated and reported for the first time the effects of relevant bioactive components on angiogenesis through different mechanisms in retinal pigment epithelial cells.<\/jats:p>","DOI":"10.1111\/jcmm.70327","type":"journal-article","created":{"date-parts":[[2025,1,4]],"date-time":"2025-01-04T03:40:57Z","timestamp":1735962057000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Upshot of Some Bioactive Compounds on Angiogenesis in Retinal Pigment Epithelial Cells"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2884-4753","authenticated-orcid":false,"given":"Serkan","family":"Sen","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Techniques, Ataturk Vocational School of Health Services Afyonkarahisar Health Sciences University  Afyonkarahisar Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2748-9702","authenticated-orcid":false,"given":"Murat","family":"Kasikci","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Mu\u011fla Training and Research Hospital  Mugla Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04482"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450121666200428103334"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.06.006"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2009.04.021"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.16757"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17902"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2012.05.009"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2012.05.012"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2006.10.009"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3797\/scipharm.0912-18"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2019.04.002"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.108999"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-658102-7.50012-9"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2011.03.012"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000101926"},{"key":"e_1_2_8_17_1","first-page":"3563","article-title":"Cigarette Smoke\u2010Induced EGFR Activation Promotes Epithelial Mesenchymal Migration of Human Retinal Pigment Epithelial Cells Through Regulation of the FAK\u2010Mediated Syk\/Src Pathway","volume":"17","author":"Park G. 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