{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:09:44Z","timestamp":1787015384035,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,5]],"date-time":"2025-01-05T00:00:00Z","timestamp":1736035200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Breast cancer (BRCA) is one of the most common malignancies and a leading cause of cancer\u2010related mortality among women globally. Despite advances in diagnosis and treatment, the heterogeneity of BRCA presents significant challenges for effective management and prognosis. Recent studies emphasise the critical role of the tumour microenvironment, particularly immune cells, in influencing tumour behaviour and patient outcomes. This study uses machine learning\u2013based methodologies to investigate the role of tumour protein D52 (TPD52) as a pivotal immune regulator in BRCA. We employed single\u2010cell RNA sequencing (scRNA\u2010seq) to characterise the immune landscape of breast tumours and identify differentially expressed genes (DEGs) associated with TPD52. Our findings indicate that TPD52 may modulate immune cell infiltration and the tumour immune landscape, impacting tumour aggression and patient survival. Furthermore, we performed in\u00a0vitro validation to elucidate the functional implications of TPD52. By integrating computational analysis with experimental validation, this research highlights TPD52's potential as a biomarker for therapeutic intervention and provides insights into its role in immune regulation within the BRCA microenvironment.<\/jats:p>","DOI":"10.1111\/jcmm.70333","type":"journal-article","created":{"date-parts":[[2025,1,5]],"date-time":"2025-01-05T22:31:53Z","timestamp":1736116313000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>TPD52<\/scp>\n                    as a Therapeutic Target Identified by Machine Learning Shapes the Immune Microenvironment in Breast Cancer"],"prefix":"10.1111","volume":"29","author":[{"given":"Jie","family":"Xia","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, National Clinical Research Center of Respiratory Disease Key Laboratory of Pulmonary Diseases of Health Ministry, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5623-7538","authenticated-orcid":false,"given":"Xudong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Thyroid and Breast Surgery Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-020-0266-x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-024-00714-6"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2020.107753"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-00449-4"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-007-0416-y"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.104683"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24032542"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-014-2006-x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.22038"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2021.1917130"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.84577"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-021-00968-x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.01.035"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2019.12.003"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-022-01569-x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-020-0265-5"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3394"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-1833"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.21037\/tcr-23-2295"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3192"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.17849\/insm-47-01-31-39.1"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1111"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-4-r41"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.239244.118"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506580102"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00795"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.844144"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btab806"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa407"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70333","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T00:47:39Z","timestamp":1746838059000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70333"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/jcmm.70333"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70333","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-11-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70333"}}